人结直肠癌干细胞标志物富含亮氨酸重复单位的G蛋白耦联受体5基因的表达及意义

摘要

目的 探讨人结直肠癌组织及外周血中干细胞标志物富含亮氨酸重复单位的G蛋白耦联受体5(lgr5)基因的表达及其与临床病理特征间的关系.方法 采用SYBR Green实时定量PCR方法检测27份结直肠癌组织及配对的正常组织中、1 7例患者及8名健康对照者外周血中lgr5mRNA的表达.应用Wilcoxon秩和检验分析lgr5 mRNA在不同组织间、临床病理参数之间的表达差异.结果 结直肠癌组织中lgr5 mRNA表达水平为1.000(0.012,496.353),高于配对正常组织的0.147(0.004,73.002),差异有统计学意义(Z=8.029,P＜0.01).结直肠癌患者外周血中lgr5mRNA表达水平为0.742(0.077,456.566),高于健康对照组的0.104(0.034,0.274),差异有统计学意义(Z=2.048,P＜0.05).结直肠癌组织lgr5 mRNA在不同性别、年龄、肿瘤原发部位、肿瘤大小、组织学类型组间表达差异均无统计学意义(P均＞0.05),但有淋巴结转移组lgr5 mRNA表达高于无淋巴结转移组,差异有统计学意义(Z=2.066,P＜0.05).结论 lgr5在结直肠癌组织及外周血中的表达上调可能参与了结直肠癌的生长及转移.%Objective To investigate the expression of stem cell marker leucine-rich repeat containing G protein coupled receptor 5 (lgr5) gene in human colorectal cancer tissues and peripheral blood and its correlation with clinical pathological characteristics.Methods The expression of lgr5 at mRNA level was detected by SYBR Green quantitative real-time polymerase chain reaction (PCR) in 27 human colorectal cancer tissues and corresponding non-cancerous tissues as well as in peripheral blood of 17 patients and eight healthy controls.The differences of lgr5 mRNA expression in different tissues and clinical pathology parameters were analyzed by Wilcoxon test.Results The expression of lgr5 at mRNA level in colorectal cancer tissues was 1.000 (0.012,496.353),which was higher than that of corresponding non-cancerous tissues 0.147 (0.004,73.002),the difference was statistically significant (Z=8.029,P＜0.01).The lgr5 expression at mRNA level in peripheral blood of colorectal cancer patients was 0.742 (0.077,456.566),which was higher than that of healthy controls 0.104 (0.034,0.274) and the difference was statistically significant (Z=2.048,P＜0.05).There were no statistically significant differences between lgr5 expression at mRNA level and gender,age,primary location of tumor,tumor size and pathological type (all P＞0.05).However,the expression of lgr5 at mRNA level in group with lymph node metastasis was higher than that in group without lymph node metastasis (Z=2.066,P＜0 05).Conclusion The up-regulation of lgr5 gene expression in colorectal cancer tissues and peripheral blood may be involved in the growth and metastasis of colorectal cancer.