南北方食管癌高、低发病区患者幽门螺杆菌感染分析

摘要

目的 比较幽门螺杆菌(Hp)感染在南北方食管癌高、低发病区之间的异同,探讨Hp感染在食管鳞癌发生过程中的作用机制.方法 选择南方食管癌高发区、低发区食管癌标本各40例,北方食管癌高发区、低发区食管癌标本各40例,另选择同地区正常食管组织标本各20例,运用实时荧光定量PCR方法扩增Hp特异基因glmM,判断食管癌组织及正常食管组织中是否存在Hp感染.结果 南北方各自食管癌高发病区Hp感染高于食管癌低发病区,差异具有统计学意义(P＜0.05).北方食管癌高发病区食管鳞癌组织和正常食管组织的Hp感染率分别高于南方,但差异无统计学意义(P>0.05).结论 Hp感染可能是食管鳞癌发生和发展的一个主要因素事件.%Objective To detect helicobacter pylori ( Hp ) infection for esophageal cancer in high-risk and low-risk areas in southern and northern China, and to analyze the role of Hp infection in esophageal squamous cell carcinoma ( ESCC ).Methods Selective 40 samples of the ESCC in high-risk and low-risk area in northern China separately, another 40 samples in high-risk and low-risk area in southern China, and 20 samples of normal esophageal were collected in similar area. The use of real time fluorescent quantitative PCR method and PCR amplification ghnM of Hp specific genes, determined whether there was Hp infection in carcinoma or normal samples. Results The infection rates of Hp in the ESCC was higher in regions of high-risk than that of in the low-risk area( P ＜ 0.05 ), whether not in northern and southern China. There was high rates of Hp infection in the ESCC and normal samples in northern China than that of in southern China, while no statistical difference was validated( P ＞ 0.05 ). Conclusion The Hp infection may be an important event of the ESCC development.