食管鳞癌组织中环氧化酶-2、P-糖蛋白的表达及临床意义

摘要

Objective To study the Cyclooxygenase -2(COX-2) and P-glycoprotein (P-gp) expression in esophageal squamous carcinoma tissues and its clinical significance .Methods The immunohistochemical method to detect COX-2 and P-gp in esophageal squamous carcinoma tissues and normal esophageal tissue expression .Results The whole set of esophageal tissue COX-2 expression positive rate was 60.0%(36/60), no expression was found in normal esophageal tissue .COX-2 ex-pression icreased as lesions infiltrating deepens , higher the degree of differentiation , lymph node metastasis and gradually in-crease (χ2 =8.140, P =0.043;χ2 =15.952, P =0.001;χ2 =13.302, P =0.001), the expression of COX-2 was associat-ed with TNM staging,III,IV expression was significantly higher in I ,II period (χ2 =17.778, P =0.001).Full set of esopha-geal tissue P-gp expression positive rate was 45.0%(27/60), normal tissue positive rate was 20.0%(12/60).The expres-sion of P-gp as the tumor infiltration depth deepening ,nodal metastasis and elevated expression (χ2 =10.649, P =0.014;χ2 =6.877, P =0.009) P-gp expression was associated with the TNM staging of esophageal cancer ,III,IV period of expression than I,II period (χ2 =5.455, P =0.020).COX-2 and P-gp in the expression of esophageal squamous carcinoma tissues were positively correlated( r =0.397, P =0.021 ).Conclusion COX-2, P-gp involved in esophageal cancer occurrence , devel-opment and transfer of multiple links .The expression of COX-2 and P-gp were positively correlated , COX-2 can regulate the expression of P-gp through some mechanism , to participate in esophageal multi-resistant adjustment .%目的：探讨环氧化酶-2（COX-2）与P-糖蛋白（P-gp）在食管鳞癌组织中的表达及其临床意义。方法选择60例食管鳞癌患者肿瘤组织与正常组织，应用免疫组织化学方法检测COX-2和P-gp在食管鳞癌组织及正常食管组织中的表达情况。结果食管癌组织COX-2表达的阳性率为60膊．0％（36/60），正常食管组织未表达。 COX-2的表达随着病变浸润的加深，分化程度的升高，淋巴结的转移而逐渐上调（χ2＝8．140， P ＝0．043；χ2＝15．952， P＝0．001；χ2＝13．302， P ＝0．001），COX-2的表达与 TNM 分期相关，Ⅲ、Ⅳ期中的表达明显高于Ⅰ、Ⅱ期（χ2＝17．778， P ＝0．001）。食管癌组织P-gp表达的阳性率为45．0％（27/60），正常组织阳性率为20．0％（12/60），差异有统计学意义（ P ＜0．01）。 P-gp的表达随着肿瘤浸润深度的加深，淋巴结的转移而表达升高（χ2＝10．649， P ＝0．014；χ2＝6．877， P ＝0．009）。 P-gp的表达与食管癌的TNM分期相关，Ⅲ＋Ⅳ期中的表达高于Ⅰ＋Ⅱ期（χ2＝5．455， P ＝0．020）。 COX-2与P-gp在食管鳞癌组织的表达呈正相关（ r ＝0．397， P ＝0．021）。结论 COX-2、P-gp参与了食管癌发生、发展、转移的多个环节，COX-2可以通过某种机制调控P-gp的表达，从而参与食管癌多药耐药的调节。