神经调节蛋白1对心肌梗死急性期大鼠心肌组织钾、钙离子通道蛋白表达的影响

摘要

Objective To investigate the effects of neural regulatory protein 1 ( NRG-1) on acute myocardial infarc-tion rats myocardium potassium , calcium ion channel protein expression .Methods Forty-five healthy SD rats were randomly divided into 3 groups , the sham operation group ( SO group ) , acute myocardial infarction model group ( AMI group ) , acute myocardial infarction rhNRG-1 intervention group (NRG-1 group), and 15 rats in each group.Establish acute myocardial in-farction model, after 24 h, took peri-infarcted myocardium and used Western blot to detect transient outward potassium chan -nel KV1.4 and L type calcium channel beta subunit ltccs beta protein expression .Results AMI group and NRG-1 group’ model was successfully established .Myocardial infarction area did not show statistical significance [(33.3 ±4.9)% vs. (35.4 ±3.6)%, P >0.05].Compared with the SD group, AMI group’s KV1.4 reduced (0.43 ±0.23 vs.0.20 ±0.17, P <0.05);and the differences of LTCCs beta showed no statistical significance (1.05 ±0.31 vs.1.00 ±0.21).Compared with AMI group, NRG-1 group (0.66 ±0.29) peri-infarcted area of myocardium ’ s KV1.4 protein expression increased sig-nificantly, LTCCs beta protein expression (0.18 ±0.11) decreased significantly , the differences were statistically significant ( t =20.95, P <0.05).Conclusion It demonstrated that the NRG-1 for acute myocardial infarction can increase KV 1.4, reduce the protein expression of LTCCs beta , which can play a role in the stability of ECG of myocardial protection .%目的：探讨神经调节蛋白1（NRG-1）对心肌梗死急性期大鼠心肌组织钾、钙离子通道蛋白表达的影响。方法45只健康SD大鼠随机分为3组，假手术组（SO组）、急性心肌梗死模型组（AMI组）、急性心肌梗死rh-NRG-1干预组（ NRG-1组），每组15只。建立急性心肌梗死模型24 h后取心脏梗死周边区心肌组织，以Western blot法检测瞬时外向钾通道KV1．4和L型钙通道β亚单位LTCCsβ的蛋白表达情况。结果 AMI组和NRG-1组模型建立成功，心肌梗死面积比较差异无统计学意义[（35．4±3．6）％ vs．（33．3±4．9）％， P ＞0．05]。与SO组比较，AMI组KV1．4降低（0．43±0．23 vs．0．20±0．17， P ＜0．05）；而LTCCsβ差异无统计学意义（1．05±0．31 vs．1．00±0．21， P ＞0．05）。与AMI组相比，NRG-1组（0．66±0．29）梗死周边区心肌组织KV1．4蛋白表达显著增加，LTCCsβ蛋白表达（0．18±0．11）显著下降，差异均有统计学意义（ t ＝20．95， P ＜0．05）。结论心肌梗死急性期给予NRG-1干预，能够上调KV1．4，下调LTCCsβ的蛋白表达，可发挥稳定心电保护心肌的作用。