糖尿病大鼠消化道的生物力学和形态重构

摘要

目的 观察链菌脲酶素(STZ)诱导的糖尿病大鼠,其消化道的形态学和生物力学特性是否发生了重构. 方法 用(STZ)复制糖尿病大鼠模型(DM)组,用注射生理盐水作为正常对照(NC)组.定期检测血糖和体重.造模成功60 d后,测量管壁的湿重、壁厚、展开角、残余应变和分析其应力应变关系. 结果 DM组大鼠食管和各个肠段的湿重与体重的比值、壁厚、展开角和内外残余应变的绝对值,明显高于NC组(P＜0.05和P＜0.01).DM组食管和结肠的周向和轴向的管壁硬度与正常对照组比较,均有统计学意义的增加(P＜0.05),而空肠表现为周向管壁硬度的增加(P＜0.05). 结论 STZ诱导的糖尿病大鼠的食管,小肠,结肠和直肠的形态学和生物力学均发生了重构,其管壁的重构可能与糖尿病病人的胃肠功能紊乱有关.%Objective To investigate the morphological and biomechanical remodelings of gastrointestinal(GI) tract in streptozotocin(STZ) induced diabetic rats. Methods The rat models were established with STZ injection and the rats in the control group were'injected with normal saline. The blood glucose and body weight were regularly measured. Sixty days after the successful modeling, the wet weight, thickness, developing angle, and residual strain of the GI tract were measured and its stress-strain relation was analyzed. Results The absolute value of ratio of the wet weight of the esaphageal and intestinal segments to the body weight, thickness, opening angle, and residual strain of inner and outer walls of GI tract was significantly higher in diabetes group than in the control group(P<0. 05 and P< 0.01). Compared with the control group, the stiffness of the esophageal and colonic walls in circumferential and longitudinal directions increased in DM group (P<0. 05) , while that of the jejunum in circumferential direction increased ( P < 0. 05). Conclusion The experimental diabetes induces the morphological and biomechanical remodeling of GI tract of rats. The remodeling of the walls of GI tract is likely related to the gastrointestinal disorders in the diabetic patients.