Objective To observe the effect of artificial fermented cordyceps mycelium (CM) on the expressions of NF-kB and TGF-βl in the cardiac muscle of streptozocin-induced diabetic rats. Methods A total of 53 healthy male SD rats, aged 8 weeks, weighted(220±20) g, were randomized into normal control group (n=10), diabetic group (n=10), and CM group [n=12, 0.6 g/(kg·d); n=11, 2. 5 g/(kg·d); n=10, 5 g/(kg·d)]. Tail vein injection with streptozotocin 45 mg/kg was adopted to produce the diabetic rat models. Diabetic rats were sacrificed after 8 weeks. The expressions of NF-κB and TGF-βl proteins and mRNA in the cardiac muscle were determined by using immunohistoche-mistry staining and reverse transcription-polymerase chain reaction (RT-PCR) method. The data were analyzed by using one-factor variance analysis. Results The expressions of NF-κB and TGF-fil proteins and mRNA in the cardiac muscle of diabetic rats were significantly increased (P<0. 05). The intervention with cordyceps mycelium could decrease the expressions of NF-κB and TGF-βl proteins and mRNA CP< 0. 05). Conclusion The changes in the expressions of NF-κB and TGF-fil in the myocardial tissue may be involved in the occurrence of diabetic cardiomyopathy. Cordyceps mycelium may play its role on myocardial protection through regulating the expressions of NF-κB and TGF-βl in the myocardium.%目的 观察人工发酵虫草菌丝体干粉(CM)对糖尿病大鼠心肌核因子-κB(NF-κB)和转化生长因子-β1(TGF-β1)表达的影响. 方法 健康雄性SD大鼠53只,鼠龄8周,体重(220±20)g.采用数字表法将大鼠随机分为正常对照组(NC,n=10)、单纯糖尿病组(n=10)、CM小剂量组[n=12;CM 0.6 g/(kg·d)灌胃]、CM中剂量组n=11;CM 2.5 g/(kg·d)灌胃]、CM大剂量组[n=10;CM 5 g/(kg·d)灌胃].单次尾静脉注射STZ(45mg/kg)造模,成模8周后处死大鼠.采用免疫组织化学法、RT-PCR法检测心肌NF-κB、TGF-β1蛋白和mRNA的表达水平. 结果 单纯糖尿病组心肌NF-κB、TGF-β1蛋白和mRNA表达均上调(P＜0.05),CM干预可下调NF-κB、TGF-β1蛋白和mRNA的异常表达(P＜0.05). 结论 心肌组织中,NF-κB和TGF-β1表达的改变可能参与了糖尿病心肌病(DC)的发生,CM可能通过调节NF-κB和TGF-β1在心肌的表达发挥对心肌的保护作用.
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