Objective To detect the expression of syndecan-1 on leukocytes in patients with type 2 diabetes mellitus. Methods Expression of syndecan-1 on subsets of leukocytes (neutiophil. Monocyte and lymphocyte) was determined by flow cytometry, and potential correlations between syndecan-1 and clinical characteristics were analyzed. Results Twenty-nine patients with type 2 diabetes mellitus (without any diabetic complications, T2DM group) and 24 age-matched healthy subjects (NC group) were enrolled. On neutrophils, the percentage of syndecan-1-positive cells was prominently higher in T2DM group than in NC group [(10. 372±1. 767)% vs (3. 988±0. 673)%] (P=0. 002). On monocytes, the difference in syndecan-1-positive cells between NC group and T2DM group was not significant ([(2. 221 ± 0. 310)% vs (2. 975±0. 553)%] (P=0. 241). On lymphocytes, the difference in syndecan-1-positive cells between NC group and T2DM group was also insignificant[(19. 520±3. 283)% vs (25. 093±3. 865)%] (P = 0. 288). In the T2DM group, the correlation between the percentage of syndecan-1-positive neutrophils and BMI was possitive (r=0. 572,P=0.004). Cooclnsion Compared with normal controls, the expression of syndecan-1 on peripheral neutrophils may increase in subjects with type 2 diabetes, and it is associated positively with BML.%目的 检测T2DM患者外周血白细胞表面syndecan-1的表达. 方法 通过流式细胞仪检测外周血白细胞亚群(中性粒细胞、单核细胞、淋巴细胞)上syndecan-1的表达,并分析syndecan-1的表达与多项临床参数的相关性. 结果 将29例无并发症的T2DM患者(T2DM组)和24名年龄匹配的健康对照(NC组)者纳入研究.在中性粒细胞,T2DM组syndecan-1阳性细胞率(10.372±1.767)％较NC组(3.988±0.673)％升高(P=0.002).单核细胞T2DM组syndecan-1阳性细胞率(2.221±0.310)％与NC组(2.975±0.553)％比较差异无统计学意义(P=0.241).淋巴细胞T2DM组syndecan-1阳性细胞率(25.093±3.865)％与NC组(19.520±3.283)％比较差异无统计学意义(P=0.288).T2DM组中,中性粒细胞syndecan-1阳性细胞率与BMI呈正相关(r=0.572,P=0.004). 结论 T2DM患者外周血中性粒细胞表面syndecan-1的表达增强,其阳性表达率与BMI呈正相关.
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