目的 研究中药扶正汤对MRL/1Pr红斑狼疮小鼠肾损害相关细胞因子γ干扰素(IFN-γ)和细胞间粘附分子-1(ICAM-1)水平的影响.方法 将MRL/1Pr模型小鼠随机分成4组:0.9％盐水对照组(A组)、西药组(B组)、中药组(C组)、中西医结合组(D组),治疗6周.取小鼠血清和肾组织匀浆应用酶联免疫法(ELISA)检测IFN-γ和ICAM-1水平.结果 3组治疗组血清中IFN-γ含量明显低于对照组(P＜0.01),各治疗组间差异无统计学意义.肾组织中IFN-γ含量中药组较对照组、西药组和中西医结合组明显减少(P＜0.01),而对照组、西药组、和中西医结合组间差异无统计学意义.对照组血清和肾组织中ICAM-1水平较高,3组治疗组ICAM-1水平均明显低于对照组(P＜0.01),中药组ICAM-1水平较低,明显低于西药组和中西药组(P＜0.01).结论 扶正汤可能通过下调细胞因子IFN-γ和ICAM-1水平,减少对肾组织的损伤,对狼疮小鼠肾炎发挥明显的治疗作用.%Objective To explore the effects of Fuzheng decoction on the expression levels of interferon-γ (IFN-γ) and intercellular adhesion molecule-1 (ICAM-1), the cytokines which are related to the kidney lesions in MRL/lPr lupus erythematosus mice. Methods MRL/lPr mice were randomly divided into four groups. Mice of group A, B, C and D were intra-gastric administrated with 0.9% saline, western medicine, traditional Chinese medicine or combination of Chinese and western medicine respectively for six weeks. Enzyme linked immunosorbent assay (ELISA)was used to measure the level of IFN-γand ICAM-1 in serum and kidney. Results There was a significant decline in serum IFN in group B, C, D compared with group A (P<0.01). No obvious difference was observed between group B, C and D. A significant decline in renal IFN content was observed in group C compared with that of group B and D (P<0.01). There was no difference in renal IFN content between group A, B and D. There was a relative higher ICAM-1 level in both serum and kidney in the control group compared with other three groups (P<0.01). There was a significant reduction of ICAM-1 level in group C compared with group B and D (P<0.01). Conclusion Fuzheng decoction may relieve the renal damage and play a role in treatment of nephritis in erythematosus lupus mice by down-regulating the IFN-γand ICAM-1 levels.
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