目的 通过对单卵双胞胎外周血T细胞的研究,寻找与银屑病发病相关的差异表达基因,进一步探讨T细胞在银屑病发病中的机制.方法 采集两对同卵双胞胎[一对双胞胎同时患病(P1、P2),另一对双胞胎一个患病一个不患病(P3、N1)]外周血,分离并扩增T细胞,采用高通量RNA测序技术筛选差异表达基因,并对筛选的基因进行GO功能分析.结果 经过分析,共注释到9 449个基因,其中P1与P2相比较在表达上无差异的基因有5 438个,P3与N1相比较在表达上存在差异的基因有2432个；差异表达基因在同时患病双胞胎中占全部表达基因的57.55％,在不同时患病双胞胎中占全部表达基因的25.74％,表明不同时患病双胞胎基因表达差异率明显高于同时患病组；银屑病组与对照组相比较在表达上存在差异的基因有105个；且与特应性皮炎组比较在表达上存在差异的基因有40个,表达上调的基因有24个.结论 在外周血T细胞中,银屑病患者存在差异表达基因,可能与银屑病患者外周血T细胞活性异常有关.%Objective To search differential expressed genes in peripheral T cells of monozygotic twins patients with psoriasis, and to further discuss the role of T cells in pathogenesis of psoriasis. Methods Differential expressed genes in peripheral T cells of twins patients with psoriasis were searched through the new generation technology of high-throughput RNA sequencing, and also done with the CO functional analysis. Results 9 449 genes were annotated after analysis, including 5 438 genes which had no difference compared with P1 and P2 in gene expression, and 2 432 genes had differences compared with P3 and N1 in gene expression. Differential expressed genes in both diseased twins accounted for 57.55% of all expressed genes, and in only one diseased twin accounted for 25.74% of all expressed genes. It was indicating that the differences in gene expression of one diseased twin were more significant than that of the same diseased twins. Compared with psoriasis group and control group, there were 105 genes that had differences in gene expression; and compared with atopic dermatitis group, there were 40 genes that had differences in gene expression. And 24 genes were up-regulated. Conclusion Many genes are differential expressed in T cells of psoriatic patients, which might play role in abnormal immune responses of T cells in psoriatic patients.
展开▼
