αvβ6-ERK直接通路在乌司他丁抑制结肠癌侵袭转移中的作用机制

摘要

Objective: To investigate the role of αvβ6-ERK direct pathway in the inhibitive effects of ulinastatin(UTI) on colon cancer. Methods: 100 cases of colon cancer were randomly divided into control and treatment group. ELISA was used to detect MMP-9/2 levels in serum and im-munohistochemistry to observe α v β 6 expression in cancer tissue; HT-29 cells were randomly divided into different groups by UTI concentration. Transwell invasion assay was applied to detect invasive ability and zymography to analyze MMP-9/2 levels. Western blot were used to detect a v p 6 expression and ERK changes. Results: UTI reduced MMP-9/2 levels in serum and α v β 6 expression in tumor tissue. Meanwhile, UTI significantly inhibited invasive ability and MMP-9/2 secretion of HT-29 cells. In addition, Western blot demonstrated that αvβ6 and p-ERK expression decreased with increasing doses of UTI. Conclusion: UTI can significantly inhibit invasion of colon cancer, and αvβ 6-ERK mediated MMP-9/2 secretion might play a key role in this process.%目的:探讨αv β 6-ERK直接通路在乌司他丁(UTI)抑制结肠癌侵袭转移中的作用.方法:100例结肠癌患者随机分为对照组和治疗组,ELISA检测血清MMP-9/2水平,免疫组织化学检测结肠癌组织αvβ6表达；HT-29细胞按UTI不同浓度分组,Transwell小室检测细胞侵袭能力,明胶酶谱检测MMP-9/2水平,Western Blot检测细胞αvβ6和ERK变化.结果:UTI可降低结肠癌患者血清MMP-9/2水平及肿瘤组织αvβ6表达强度；UTI可抑制HT-29细胞侵袭能力及MMP-9/2分泌水平,并显著下调αvβ6表达和ERK磷酸化水平.结论:UTI可显著抑制结肠癌的侵袭浸润,αvβ6-ERK 直接通路介导的MMP-9/2分泌可能在其中发挥重要作用.