磷酸化雌激素受体alpha在肝癌组织中的表达

摘要

目的:研究磷酸化雌激素受体α(p-ERα)在肝癌组织中的表达,探讨其与肝癌生物学特征及患者预后的关系.方法:采用免疫组织化学法检测77例肝癌组织中p-ERα中的表达,采用Westernblot和RT-PCR检测肝癌组织、癌旁组织以及正常肝组织中p-ERα的蛋白表达及ERα mRNA表达情况.结果:肝癌组织中p-ERα的表达阳性率为48%(37/77).p-ERα在肝癌组织中的表达低于癌旁组织及正常肝组织.p-ERα的表达与肝癌包膜(X2=13.487,P＜0.01)、镜下癌栓(X2=5.751,P＜0.05)有关.Kaplan-Meier生存分析显示,p-ERα阳性与P-ERα阴性患者的总体生存率(51.4%VS 37.5%,X2=1.491,P=O.22)与无瘤生存率(56.8%VS60%,X2=0.300,P=O.58)差异无统计学意义.结论:P-ERα对肝癌的预后可能没有影响,但对肝癌患者的生物学特性有一定的影响.%Objective: To study the expression of Phosphorylated-Estrogen Receptor α (p-ER α ) in HCC, and the relationship between the expression of p-ER α and the prognosis of HCC patients. Methods: Immunohistochemistry was used to detect the expression of p-ER α in 77 cases with HCC, and westernblot and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expression of p-ER α protein and ER α mRNA in HCC, their corresponding adjacent liver tissues and normal liver tissues. Results: The positive rate of p-ER α in HCC tissue specimens was 45％ (35/77). The expression of p-ER α was lower in HCC tissue than in paired paracarcinomatous tissues and normal liver tissues. The expression of p-ERα was related with capsule ( x 2=13.487, P＜0.01 ) and microvascular invasived( x 2=5.751, P＜0.05)by comparing the relationship between p-ER α and HCC pathological character. Kaplan-Meier survival analysis revealed that there had no significant difference between the p-ER α positive group and the p-ER α negative group in the overall survival rate (51.4％vs37.5％, x 2=1.491, P=0.22) and disease-free survival rate (56.8％vs60％, x2=0.300, P=0.58). Conclussions: The p-ER α may not affect the prognistic of HCC patients, which may have little role in the tumor biological features.