Objective:SFRP2 gene is a member of the SFRPs family.The gene is located on chromosome 4q31.3 with 3 exons and 2 introns and first exons have higher density near the island of CpG.Many studies showed that the methylation level of SFRP2 gene and colon cancer,esophageal cancer,gastric cancer and other tumor occurrence relating to,development and prog nosis.This study aims to study the clinical characteristics of CpG SFRP2 promoter island hypermethylation in colorectal cancer and whether there is a certain correlation.Methods:by matrix assisted laser desorption ionization time of flight mass spectrometry for detecting specific CpG island methylation.Methylation status of SFRP2 promoter by Sequenom EpiTYPER was detected in 20 cases of normal tissue of colorectal cancer and tumor tissues.Results:Our study using multiple linear regression analysis in tumor tissue of SFRP2 methylation at promoter Ⅰ and Ⅱ,found that SFRP2 promoter methylation and clinical features of.SFRP2_01_CpG_5 significantly correlated (P=0.018),SFRP2_02_CpG_5 (P=0.018) associated with the location of the tumor,SFRP2_02_CpG_6,7,8,9(P=0.039) and the number of lymph node metastasis of.SFRP2_01_CpG_1.2(P=0.043),SFRP2_02_CpG_16 (P=0.044) correlated with tumor size.Conclusion:we from epigenetic aspects of its promoter CpG methylation level and colorectal cancer clinical and pathological features,found a correlation between clinical and pathological features of colorectal cancer and CpG methylation in its promoter,suggesting that the SFRP2 promoter may be at this stage of colorectal cancer and future biological genetics the progress of the potential surface markers.%目的:研究SFRP2启动子中CpG岛高甲基化与结直肠癌临床特点之间的相关性.方法:通过基质辅助激光解吸附电离飞行时间质谱分析技术检测特定CpG岛甲基化程度,采用Sequenom EpiTYPER技术检测20例结肠癌中正常组织和肿瘤组织中SFRP2启动子甲基化状态.结果:SFRP2_01_CpG_5 (P=0.018)、SFRP2_02_CpG_5 (P=0.018)与肿瘤位置相关,SFRP2_02_CpG_6、7、8、9 (P=0.039)与淋巴结转移个数有关.SFRP2_01_CpG_1.2(P=0.043)、SFRP2_02_CpG_16(P=0.044)与肿瘤直径大小相关.结论:启动子CpG位点甲基化水平与结直肠癌的临床及病理特点有相关性,表明SFRP2启动子可能是结直肠癌的现阶段和未来进展的潜在的表观遗传学的生物学标志物.
展开▼
