不稳定心绞痛与血小板CD63、CD62P及血浆PAI-1、C-RP的关系

摘要

目的 探讨不稳定心绞痛(UAP)血小板胞浆内α-颗粒上的膜糖蛋白(CD62P)及溶酶体膜糖蛋白(CD63)与血浆纤溶酶原活物-1(PAI-1)、C-反应蛋白(C-RP)的关系，并揭示其在UAP发生及发展中的意义。方法 应用流式细胞术检测59例UAP患者血小板表面活性标志蛋白CD63、CD62P，同时检测血浆PAI-1及C-RP水平。结果 ①与对照组比较，UAP患者CD63、CD62P、PAI-1及C-RP水平明显增高(P＜0.05或P＜0.01)；②CD63、CD62P与PAI-1及C-RP呈正相关；③CD63、CD62P及PAI-1、C-RP水平在心绞痛发作状态比发作前及发作后状态明显增高(P＜0.05或P＜0.01)；④随着UAP患者Braunwald分级及冠状动脉病变支数的增加，CD63、CD62P及PAI-1、C-RP水平均有增加趋势。结论 血小板活化、纤溶机制及炎症反应在UAP的发生及发展中共同作用；这三者可能成为UAP患者病情程度及预后判别的一个良好指标。%Objective To explore the correlation between platelet activationfactor CD63、CD62P and plasma plasminogen activator inhibitor-1(PAI-1), C-reactive protein(C-RP)in patients with unstable angina(UAP).Methods Platelet activation factor CD63, CD62P were detected by flow cytometry,and plasma PAI-1, C-RP were measured by enzyme-immeoassay in UAP.Results ①Platelet activation factor CD63、CD62P and plasma PAI-1 activity, serum C-RP concentration were significantly higher than those of the normal subjects and the stable angina(SAP)subjects(P＜0.05 or P＜0.01);②There were correlation between platelet CD63、CD62P and plasma PAI-1, C-RP;③The increase of all indicators were much obvious higher in angina attack than before angina attack and in relief(P＜0.05 or P＜0.01);④There were positive correlation between all indicators and the degree of angina and coronary stenosis.Conclusions Platelet activation,PAI-1 activity, C-RP involved in the development of UAP,and predicted the prongnosis of UAP.