目的 观察大承气汤对脓毒症大鼠肝脏p38丝裂原活化蛋白激酶(p38MAPK)表达水平的影响,进一步揭示大承气汤治疗脓毒症的机制.方法 将60只健康雄性SD大鼠随机分为假手术组(n=20)、脓毒症组(n=20)、大承气汤组(n=20),应用大鼠盲肠结扎穿孔(CLP)复制脓毒症模型.假手术组开腹轻扰肠管后关腹,脓毒症组、大承气汤组均为脓毒症模型.大承气汤组在术前2 h和术后每4 h予以大承气汤灌胃.分别于术后2、6、12、24 h每组取5只大鼠,腹主动脉采血,用ELISA方法 检测大鼠血浆IL-6、TNF-α浓度,取肝组织进行免疫组化检测及观察p38MAPK表达.结果 与脓毒症组比较,大承气汤组大鼠肝脏p38MAPK表达水平受到抑制,炎症因子释放减少(P<0.05).结论 大承气汤对脓毒症大鼠的肝脏产生保护作用,可能是由于抑制大鼠肝脏p38MAPK表达来实现的.%Objective To provide the further evidence for the mechanisms of the Dachengqi decoction treatment of sepsis by observing the effect of Dachengqi decoction on the p38 mitogen -activated protein kinase (p38MAPK) in septic rats' liver. Methods 60 SD rats were divided into three groups randomly: sham - operation group, septic group and Dachengqi decoction intervention group. The septic models were made by cecal ligation and puncture ( CLP). Dachengqi decoction group was lavaged with Dachengqi decoction, while the sham - operation group and septic group were lavaged with the equivalent saline at the same time. Five rats were killed randomly in each group at the point of 2, 6, 12, 24 h after CLP. Serum samples were taken after centrifugation and the level of TNF - α, IL - 6 were detected by ELISA. The hepatic tissues were taken for the test of p38MAPK level. Results Compared with sham operation group, the level of p38MAPK in septic group increased significantly at the point of 6, 12, 24 h after CLP(P < 0. 05 ). Compared with septic group, the level of p38MAPK in Dachengqi decoction intervention group decreased significantly at the point of 12,24 h (P <0.05). Conclusion The mechanism of Dachengqi decoction treatment for sepsis probably includes the inhibition of p38MAPK expression in liver.
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