AIM: To evaluate the antibacterial effect of nano-silver, Ag-embedded nano-Ti02 and nitrogen-doped nano-Ti02 against E. Faecalis. METHODS: E. Faecalis was inoculated in MBECTM P&G Assay to construct an E. Faecalis infection model. E. Faecalis infection on MBEC? P&C Assay was observed by scaning electron microscope. The minimum inhibitory concentration ( MIC) and minimum biofilm eradication concentration ( MBEC) were detected by serial dilution method using MBEC? P&G Assay. RESULTS; E. Faecalis biofilm formed on MBEC? P&G Assay. MIC and MBEC of nano-silver against E. Faecalis were 62.5 mg/L and 1,000 mg/L respectively. MIC of Ag-embedded nano-TiO2 against E. Faecalis was 64 g/L. MBEC of Ag-embedded nano-Ti02, MIC and MBEC of nitrogen-doped nano-Ti02 could not be detected in this study. MIC and MBEC of sodium hypochlorite were 6.56 g/L and 13.13 g/L. CONCLUSION; Nano-silver has strong antibacterial effect on E. Faecalis and can eradicate E. Faecalis biofilm. E. Faecalis shows resistance against Ag-embedded nano-TiO2 and nitrogen-doped nano-TiO2.%目的:研究纳米银、载银纳米二氧化钛、掺氮纳米二氧化钛对粪肠球菌的抗菌作用.方法:将粪肠球菌在MBECmTM P&G Assay中培养24h,构建粪肠球菌感染模型,扫描电镜观察MBECTM P&G Assay上粪肠球菌感染情况.应用MBECTMP&G Assay以连续稀释法检测纳米银、载银纳米二氧化钛、掺氮纳米二氧化钛的最小抑菌浓度(MIC)和最小生物膜清除浓度(MBEC).结果:扫描电镜下见粪肠球菌在MBECTM P&G Assay 上形成生物膜.纳米银对粪肠球菌的MIC和MBEC分别为62.5 mg/L和1 000 mg/L,载银纳米二氧化钛MIC为64 g/L,但在所用浓度范围内均不能清除生物膜细菌,即未测得MBEC；掺氮纳米二氧化钛的MIC、MBEC均未测得；次氯酸钠的MIC和MBEC分别为6.56 g/L和13.13 g/L.结论:纳米银对粪肠球菌有较强抗菌作用,能清除粪肠球菌生物膜；粪肠球菌对载银纳米二氧化钛和掺氮纳米二氧化钛有较强抵抗力.
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