精氨酸酶在大鼠实验性牙周炎牙周组织中的表达变化

摘要

AIM; To investigate the expression of arginase (Arg) in periodontal tissues at different stages ofrndisease progression in a rat experimental periodontitis model. METHODS: Foiirty male Wistar rats were fed with high sucrose diet. Silk was ligated around the cervix of right first maxillary molars to induce periodontitis ( experimental group). The left first maxillary molars remained untreated to serve as the control. Ten rats were sacrificed at the end of week 1,2,4 and 6. Expression of Arg in periodontal tissues at different stages was detected with immunohistochemistry staining and image quantitative analysis. RESULTS; Arg was scarcely expressed in normal periodontal tissues and was mainly expressed in macrophages and endothelial cells in the experimental groups. The Arg expression increased with the ligation time, reached peak at week 4, and decreased afterwards. CONCLUSION; Arg expression is positively re-lated with the degree of periodontal inflammation.%目的:观察精氨酸酶(arginase,Arg)在不同时期、不同严重程度大鼠实验性牙周炎牙周组织中表达的变化,探讨Arg与牙周炎的相关性.方法:选用40只Wistar雄性大鼠,每只大鼠均用丝线结扎右下颌第一磨牙(实验组),左下颌第一磨牙不做处理(对照组),喂以高糖饮食.分别于结扎后1、2、4、6周,经临床和组织学(HE染色)观察确认大鼠牙龈炎、不同程度牙周炎模型建立成功后,取大鼠下颌骨连同受试牙和牙周组织,制备切片,免疫组化染色法检测正常对照组、牙龈炎组、不同程度牙周炎组大鼠牙周组织中Arg的表达,并进行图像定量分析.结果:在正常牙周组织中,偶有表达；而各实验组牙周组织中Arg主要在巨噬细胞和血管内皮细胞中表达,其表达阳性细胞数随炎症程度加重而增加,结扎4周(重度牙周炎)时达到高峰,到慢性牙周炎时期(结扎6周),表达较急性期有所降低,各组间比较,差异均有统计学意义(P＜0.05).结论:牙周组织内Arg的表达与牙周炎炎症程度呈明显正相关.