LPS刺激对体外培养人牙周膜细胞产生IL-8和MCP-1的影响

摘要

AIM:To investigate the effects of LPS stimulation on the expression of IL-8 and MCP-1 of human periodontal ligament cells (HPDLCs) cultured in vitro.METHODS:HPDLCs were cultured and identified.MTT method was used to exam the viability of the cells stimulated by LPS at different concentrations.Enzyme linked immunosorbent assay (ELISA) was used to detect IL-8 and MCP-1 concentration in the cell culture supernatant after stimulation by 10 μg/mL of LPS for4 h,8 h,12 h and 24 h respectively.RESULTS:Stimulation of LPS at 10 μg/mL within 24 h showed no significant effects on the morphology and proliferation of HPDLCs.ELISA revealed that the concentration of IL-8 and MCP-1 increased from 4 h to 24 h in a time dependent manner after LPS stimulation (vs the corresponding negative controls P ＜ 0.05).CONCLUSION:LPS can stimulate the production of IL-8 and MCP-1 of HP-DLCs and HPDLCs have the potential of immuno activity.%目的:检测脂多糖(LPS)刺激对体外培养人牙周膜细胞分泌炎症趋化因子IL-8和MCP-1改变的影响.方法:组织块法原代培养人牙周膜细胞,MTT法观察不同浓度LPS对其增殖的影响;取第4代牙周膜细胞在10 μg/mL LPS刺激条件下进行培养,分别于培养后4、8、12、24 h用酶联免疫吸附(ELISA)法检测牙周膜细胞培养上清中IL-8和MCP-1的含量;结果采用单因素方差进行统计分析.结果:10 μg/mL LPS刺激可使牙周膜细胞的增殖速度略减低,刺激后24 h内细胞形态和数量未见明显变化.ELISA结果显示LPS刺激后牙周膜细胞在10 μg/mL培养上清中IL-8和MCP-1含量均明显高于对照组(P＜0.05);且两种蛋白含量均随培养时间延长而增加(P＜0.05),呈时间依赖性.结论:10μg/mL LPS刺激可使牙周膜细胞培养上清中IL-8和MCP-1含量呈时间依赖性增加,提示牙周膜细胞具有免疫调节功能.