组胺及组胺受体1在大鼠牙周炎发病中表达差异的研究

摘要

AIM:To investigate the expression of histamine and histamine receptor 1 (HR1 )in rat gingival crevicular fluid (GCF)and gingival tissues.METHODS:40 adult SD rats were randomly divided into 2 groups(n=20):The periodontitis models were established by ligation technique and the rats in control group received no treat-ment.After 8 weeks,Histamine in GCF was detected by ELISA.HRI mRNA expression in gingival tissues was detec-ted by QT-PCR.RESULTS:The level of histamine in GCF in the periodontitis group (35.47 ±3.908)μg/L was high-er than that in control group (19.77 ±3.832)μg/L(P<0.05).HRI mRNA in gingival tissues of periodontitis group was lower than that of the control group (P<0.05).CONCLUSION:Upregulation of histamine in GCF and downregu-lation of HRI in gingival tissues may play important roles in pathogenesis of periodontitis.%目的：研究组胺及组胺受体1（HR1）在牙周炎发病机制中的作用。方法：将40只雄性SD大鼠随机分为实验组和对照组（每组20只），其中实验组采用正畸丝结扎法建立牙周炎模型，对照组不作任何处理；建模8周后，分别采用ELISA法检测各组龈沟液中组胺含量，荧光定量PCR法检测各组牙龈组织中HR1 mRNA的表达水平。结果：牙周炎组龈沟液中的组胺浓度（35．47±3．908）μg／L 高于正常对照组（19．77± 3．832）μg／L（P＜0．05）；牙周炎组牙龈组织中的HR1－mRNA表达水平低于正常对照组（P＜0．05）。结论：组胺及HR1对牙周炎的发病均有一定调控作用。