通光散汤对小鼠哮喘模型气道高反应性和气道炎症的影响

摘要

To investigate the effects of a Chinese traditional medicine, Tong-guang-san ( TGS) , on airway hyperresponsiveness in asthmatic mouse models. Methods Thirty five BALB/c mice at 6 weeks of age were randomized into 3 groups. The mice of model group and experimental group were sensitized with ovalbumin (OVA) by intraperitoneal injection on day 0, 7 and 14, then challenged with OVA by intranasal administration on day 28, 29, 30 for asthmatic models. From on day 15, the mice of the experimental group were administered TGS (0. 72 Ml, about 0. 04 g crude drug) via intragastric gavage once daily for 14 days. The mice of control group received saline. Bronchial hyperresponsiveness ( BHR) was examined 48 hours after the final challenge on day 32 with a whole body plethysmography (WBP) system. Bronchoalveolar lavage fluid (BALF) and histoiogical changes in the airways were examined on day 32. The data were analyzed statistically with one-way analysis of variance (ANOVA) using SPSS package (version 13.0). Results Airway resistance of the mice in the experimental group was significantly decreased compared with that of the asthma groups (P < 0. 05 ). In asthma group, the Eos percentage in BALF were 70% but those cells were scarcely seen in the normal group (0.08%). Eos in BALF in the experimental group were decreased markly compared with that in the asthma group (P < 0. 05 ). The histological examination of the lungs from the asthmatic animals revealed characteristic inflammatory cell infiltration in the peribronchial regions and around the submucosal blood vessels while those of experiment group displayed less inflammation and improved mucous edema and epithelial lesions of the bronchi and bronchioles. Conclusions TGS can significantly reduce airway hyperresponsiveness of the asthmatic mice.%目的 观察通光散对小鼠哮喘模型气道反应和气道炎症的影响.方法 35只6周龄BALB/c小鼠随机分为哮喘模型组、正常对照组和药物实验组.模型组和药物组以鸡卵白蛋白(OVA)致敏、激发；药物组在最后一次致敏后每天灌胃给予通光散汤0.72 mL(相当于0.04 g生药)；对照组以等体积的NS代替OVA致敏、激发.末次激发48 h后处理小鼠:无创法测定小鼠的气道高反应性,观察气道阻力变化；支气管肺泡灌洗液(BALF)行细胞学分类；观察肺组织的病理变化.结果 ①药物组小鼠气道阻力的变化与模型组相比明显下降,差异显著(P＜0.05)；②药物组BALF白细胞总数和Eos(％)与模型组相比明显降低(P＜0.05).③模型组小鼠肺脏组织支气管、血管黏膜下和周围肺组织有明显的炎症细胞浸润,大量炎症细胞向支气管和血管迁移,上皮细胞部分有脱落,部分可见黏液栓,血管壁明显水肿；治疗组小鼠肺组织炎性细胞浸润和管腔黏液分泌情况较模型组明显减轻,气道粘液的分泌量得到明显的控制.结论 通光散汤对小鼠哮喘模型气道高反应性和气道炎症有显著抑制作用.