Apocynin对缺血再灌注后急性肾损伤的保护作用

摘要

Objective To investigate the protective effect of Apocynin on acute renal injury after ischemia/reperfusion (I/R).Methods Acute renal ischemia/reperfusion injury in male SD rats was made by cutting right kidney and clamping left renal artery.SD rats were randomly divided into groups (sham-operated group,I/R group,I/R +Apocynin groups).Serum creatinine was assayed by biochemistry.Renal morphology changes were observed by light microscope.The amount of monocyte were observed by immunohistochemistry.MDA was assayed by thiobarbiturate.GPx was assayed by DTNB.IL-1 was assayed by Enzyme-linked immunoadsordent assay.Result Compared with shamoperated group,Scr levels were significant increased in I/R group (P ＜ 0.05),kidneys displayd significant histology changes in I/R group(P ＜ 0.05),the amount of monocyte was significant increased in I/R group (P ＜ 0.05),MDA and IL-1 were significant increased in I/R group (P ＜ 0.05),GPx was significant decreased in I/R group (P ＜ 0.05).Compared with I/R group,Scr was decreased in I/R + Apocynin groups,the amount of monocyte was decreased in I/R +Apocynin groups(P ＜0.05),MDA and IL-1 were decreased in I/R + Apocynin groups(P ＜0.05).GPx was increased in I/R + Apocynin groups (P ＜ 0.05).Conclusions Apocynin protect kidney against ischemia/reperfusion and accelerate recovery after acute renal ischemia/reperfusion injury,which may associate with the anti-oxidation and anti-inflammation%目的 观察Apocynin对急性缺血再灌注肾损伤(I/R)大鼠模型的保护作用并探讨其机制.方法 采用雄性SD大鼠制备急性缺血再灌注肾损伤模型,用不同浓度(5 mg/kg、10 mg/kg、20 mg/kg) Apocynin干预,同时设定假手术组、手术对照组,生化法测定血肌酐值,光镜下观察肾组织形态学变化并进行肾小管病变计分,免疫组织化学检测肾组织单核巨噬细胞计数,硫代巴比妥酸比色法测定肾组织匀浆丙二醛(MDA),改良DTNB显色法测定血清谷胱甘肽过氧化物酶(GPx),ELISA法测定血清白介素1(IL-1).结果 与假手术组相比,手术对照组血肌酐明显升高(P＜0.05),肾小管计分明显升高(P＜0.05),肾组织巨噬细胞数目增加(P＜0.05),血清MDA、IL-1升高(P＜0.05),GPx下降(P＜0.05)；与手术对照组相比,各Apocynin干预组血肌酐下降(P＜0.05),肾小管计分降低(P＜0.05),肾组织巨噬细胞数目减少(P＜0.05),血清MDA降低(P＜0.05),IL-1降低(P＜0.05),GPx含量升高(P＜0.05).结论 Apocynin可以降低急性缺血再灌注肾组织氧化应激水平,抑制炎症反应,减轻肾功能损伤程度.