罗汉果和熟地增加小鼠造血干细胞的数量和功能

摘要

Objective To investigate the influence of Siraitia Grosvenori and Rehmannia Glutinosa on the Hematopoietic stem cells proliferation and function .Methods Cells from the peripheral blood , spleen and bone marrow of mice were stained with indicated antibodies , and analyzed by flow cytometry .Mice were divided 3 groups:control group, Siraitia Grosvenori treatment group and Rehmannia Glutinosa treatment group .After 4.5Gy IR treatment, mice divided 4 groups: control group, 4.5Gy IR treatment and feed with normal food, 4.5Gy IR treatment and feed with Siraitia Grosvenori and 4.5Gy IR treatment and feed with Rehmannia Glutinosa for 1 month.Results Mice fed with Siraitia Grosvenori and Rehmannia Glutinosa decreased the percentage of B cells and increased the percentage of M cell .For HSCs, the number of HSCs was increased , especially the number of LT-HSCs.After 4.5Gy IR treatment, mice fed with Siraitia Grosvenori and Rehmannia Glutinosa increase the number of HSCs , and increased the percentage of M cells . Conclusion Siraitia Grosvenori and Rehmannia Glutinosa promote the hematopoietic stem cells and progenitor cells proliferation and function and recover the damage that caused by IR treatment .%目的：罗汉果和熟地具有增强机体免疫力，延年益寿的作用。本文研究罗汉果和熟地对造血干细胞的影响。方法各组小鼠连续喂养罗汉果或熟地3个月，流式细胞仪测量小鼠的外周血、脾脏和骨髓中免疫细胞和造血干细胞的变化；小鼠用半致死剂量的放射线照射后，连续喂养罗汉果或熟地1个月后，流式细胞仪测量罗汉果或熟地对于免疫细胞和造血干细胞损伤修复的作用。结果分析检测结果发现，与对照组相比，长期喂养罗汉果或熟地的小鼠骨髓和外周血中B细胞的比例降低，粒细胞的比例增加，T细胞没有明显变化；骨髓中造血干细胞的数量增加，尤其是长期造血干细胞数量增加显著。半致死剂量的放射线照射后，罗汉果或熟地喂养1个月，小鼠的粒细胞和T细胞都有明显的改善，造血干细胞的数量明显增加。结论罗汉果或熟地长期服用会增加小鼠造血干细胞的数量和功能，促进了辐射所致的骨髓抑制小鼠的造血干细胞的恢复。