SHR 大鼠前额叶、纹状体突触体的提取方法

摘要

Objective To introduce an improved extraction method of prefrontal cortical and striatal synaptosomes from SHR rat. Methods Synaptosomes were prepared from SHR rat brain tissue by Percoll density gradient centrifugation.Transmission electron microscopy was used to assess the morphology and structural integrity of the synaptosomes.Results The obtained synaptosomes showed oval structures surrounded by an intact membrane.Presynaptic components contained one or more mitochondria and a large number of synaptic vesicles.The synaptic clefts were clearly visible, and prominent part of the characteristic compact structure was clear, complete and with higher electron-density. The synaptosome presynaptic membrane, synaptic cleft, and postsynaptic membrane were well preserved, and the synaptosomes were densely distributed, showing typical morphological characteristics of synaptosomes.Conclusions The results of our study improved the traditional preparation method and provide a less time-consuming, highly productive protocol for preparation of structurally typical and intact synaptosomes, suitable for further research on neuroscience and neurological diseases.%目的：介绍SHR大鼠前额叶、纹状体突触体的提取方法。方法采用Percoll密度梯度离心法制备突触体，透射电镜观察其形态和结构完整性。结果本实验所获取的突触体形态上呈连续膜封闭的椭圆结构，周围有完整的膜包围；突触前成分可见一个或多个线粒体和大量突触小囊泡；突触间隙清晰可见，且突触后部分特征性的致密部结构完整，形态清晰，密度较高。突触体突触前膜、突触间隙、突触后膜保存完好，突触体分布密度较高，具有典型的突触体形态结构特征。结论本实验提供了一种耗时少，产出率高，结构完整、形态典型且可运用于精细化研究的突触体制备新技术，补充了传统技术的不足，为神经系统疾病的研究提供技术支持。