强志组方对多发性抽动症复合恐惧行为大鼠的影响

摘要

目的：探究强志组方治疗多发性抽动症（ TS）复合恐惧行为的作用机制。方法腹腔注射亚氨基二丙腈（ IDPN）建立TS大鼠模型后，进一步声电刺激建立TS复合恐惧大鼠模型，给予不同药物灌胃治疗后，采用旷场试验、行为学检测大鼠行为变化，高效液相法（ HPLC）检测脑组织中多巴胺（ DA）的含量，免疫组织化学法检测酪氨酸羟化酶（ TH）的含量，RT-PCR检测TH mRNA 的表达。结果与正常对照组相比，模型对照组刻板行为、运动行为增加，冻结时间延长，脑组织中 DA、TH、TH mRNA 含量无明显变化；与模型对照组相比，强志组方组刻板行为、运动行为减少，冻结时间缩短，脑组织中DA、TH、TH mRNA 含量减少。结论强志组方可改善模型大鼠抽动复合恐惧行为，其作用机制可能是通过下调TH mRNA表达，降低TH含量，减少DA合成实现的。%Objective To explore the functional mechanism of a Chinese medicine compound “Qiangzhizufang”on rat model of Tourette syndrome ( TS) combined with fear.Methods The rat model of TS combined with fear was established by intraperitoneal injection of 3,3’-iminodipropionitrile (IDPN) combined with acoustic stimulation.After giving different drug lavage treatment, the changes of behavior of the rat models were assessed by field test and behavior test.The content of DA, TH and TH mRNA in the brain tissue was detected by HPLC, immunohistochemistry and RT-PCR, separately.Results Compared with the normal control group, stereotyped behavior and exercise behavior were increased, freezing time prolonged, but the content of DA, TH and TH mRNA in the brain tissue were not obviously changed in the model control group.Compared with the model control group, the stereotyped behavior and exercise behavior were decreased, content of DA, TH and TH mRNA in the brain tissue was decreased in the “Qiangzhizufang” group. Conclusions The Chinese medicine compound“Qiangzhizufang” can improve the behavior in rat models of TS combined with fear.This effect may be realized through down-regurating TH mRNA expression, reducing the content of TH, and reducing the dopamine synthesis.