节律基因hClock及hBmal1对胃癌化疗敏感性的影响

摘要

目的：探讨节律基因hClock、hBmal1对胃癌细胞SGC-7901化疗敏感性的影响。方法：体外持续黑暗条件下培养SGC-7901，用实时定量PCR法检测两种主要的节律基因hClock、hBmal1在SGC-7901中不同时间点的表达，分别在节律基因hClock、hBmal1表达的高峰及低谷给予化疗药物多西他赛，CCK-8法检测化疗药物对肿瘤细胞的抑制率。结果：实时定量CR结果显示节律基因hClock、hBmal1在胃癌细胞SGC-7901中不同时间点表达不同，存在时相性波动，表达高峰在20：00，而表达低谷在08：00。CCK-8法显示在节律基因hClock、hBmal1表达高峰时，多西他赛对肿瘤细胞的抑制率低于其在低谷时的抑制率。结论：节律基因hClock、hBmal1过表达可以降低胃癌细胞SGC-7901对多西他赛的敏感性。%Objective:To study the influernce of circadian genes hClock and hBmal1 on the chemosensitivity of SGC-7901 cells. Methods: SGC-7901 cells were cultivated under continuous darkness in vitro.The expression levels of the two main circadian genes hClock and hBmal1 at the different time were determined by real-time polymerase chain reaction(PCR). Docetaxel was administered at the peak and nadir time point respectively. The inhibition of SGC-7901 cell proliferation was measured using a CCK-8 kit. Result:The expression of circadian genes hClock and hBmal1 varied at different times, as shown by real-time PCR. The expression of circadian genes hClock and hBmal1 showed Phase oseillation. The maximum expression of hClock and hBmal1 mRNA was at 20:00. whereas their minimum expression was at 08:00. The inhibition ratio of docetaxel to SGC-7901 cells at the maximum expression of hClock and hBmal1 genes was lower than that at the minimum expression. Conclusion:Circadian Genes hClock and hBmal1 can reduce the drug sensitivity of SGC-7901 cell line to docetaxel in vitro.