IL-15对树突状细胞激活的T淋巴细胞免疫反应的作用观察

摘要

目的：观察IL-15和IL-2对树突状细胞疫苗(dendritic cells,DC)激活的淋巴细胞免疫反应的作用.方法：取健康小鼠的脾淋巴细胞,在体外采用肿瘤抗原负载的DC疫苗与脾淋巴细胞混合后,分别加入IL-15或IL-2培养7d.采用流式细胞术检测淋巴细胞免疫表型的变化；使用酶联免疫斑点实验(ELISPOT)检测分泌干扰素(interferon,IFN)-γ的细胞数量；利用51Cr释放法检测淋巴细胞对肿瘤细胞的杀伤活性.结果：IL-15联合DC疫苗增强了T细胞反应,包括淋巴细胞免疫表型和功能的改变.结论：与IL-2相比,IL-15能更好的增强DC疫苗诱发的免疫反应,为IL-15与DC疫苗的联合应用提供了一定的依据.%Objective: This study aimed to observe the ability of interleukin-15 (IL-15) or interleukin-2 (IL-2) to act as a potent adjuvant for a dendritic cell (DCs)–directed vaccine strategy to activate T-cell responses in vitro. Methods: Splenic lymphocytes from naïve mice were prepared. Then, bone marrow-derived DCs (BMDCs) were pulsed with tumor antigens and cultured with the splenocytes in the presence of IL-15 or IL-2 for 7 days. The activated T lymphocytes were examined by flow cytometric analysis, interferon-enzyme-linked immunospot, and cytotoxicity assays. Results: IL-15 resulted in an augmentation of T-cell responses following immunizations with BMDCs. The enhanced response was based on the phenotype characteristics of the lymphocytes and their functions. Conclusion: IL-15 may act as a potent adjuvant for the DC-directed vaccine strategy.