合并多囊卵巢综合征产妇胎盘肥胖相关基因多态性研究

摘要

Objective:To study the polymorphism of fat mass and obesity associated gene(FTO) rsl421085 (C/T) in placenta tissues from patients with polycystic ovarian syndrome (PLOS),in order to explore the risk of PCOS and relevant diseases in offspring of PCOS patients. Methods: A total of 28 cases of PCOS and 27 healthy volunteers were enrolled. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELF) and gene sequencing were used to detect the single nucleotide polymorphism(SNP) of FTO rsl421085 (C/T). Results; The allele frequency of FTO rsl421085 (C/T) was significantly different between study group and control group (P = 0. 0072). The frequency of genotype was significantly different between the two groups (P = 0. 0285). Conclusions; The SNP of FTO rsl421085(C/T) variation rate is significantly increased in PCOS patients. It reveals that the offspring of PCOS women may have higher risk in suffering from PCOS and relevant diseases.%目的:研究多囊卵巢综合征(polycystic ovarian syndrome,PCOS)患者胎盘肥胖相关基因(fat mass and obesity associated gene,FTO) rs1421085(C/T)的单核苷酸多态性(single nucleotide polymorphism,SNP),探讨PCOS患者子代再次发生p-COS及相关疾病的风险.方法:运用聚合酶链反应-限制性片段长度多态性(Polymerase chain reaction-restriction fragment length polymorphism,PCR-RELF)分析及基因测序的方法,在26例PCOS患者(研究组)和27例健康志愿者(对照组)的胎盘组织中,检测FTO基因rs1421085(C/T)位点的SNP.结果:研究组胎盘FTO基因rs1421085 (C/T)等位基因频率与对照组比较有显著差异(P=0.0072).两组基因型频率相比也有显著差异(P=0.0285).结论:PCOS患者胎盘组织中FTO基因rs1421085 (C/T)位点SNP变异的发生率较正常人群高,PCOS患者后代再次发生PCOS及相关疾病的风险可能会增加.