可注射性纳米材料与兔成骨细胞的生物相容性

摘要

目的:将可注射性纳米材料与兔成骨细胞复合构建可注射性组织工程骨,观察其体外实验的生物相容性.方法:取16周龄、体质量约1.5 kg的新西兰大耳白兔双股骨大转子部和胫骨干骺端骨髓4～5 mL,将传至第三代的骨髓基质干细胞以成骨条件培养基定向诱导培养,获取成骨细胞.将成骨细胞与可注射性纳米材料体外复合培养,用噻唑蓝法测定细胞活性,检测碱性磷酸酶(ALP)活性,并行激光共聚焦显微镜及扫描电镜观察.结果:可注射纳米材料组的成骨细胞保持正常的分裂增殖速度.可注射纳米材料组ALP活性值为2.135±0.085,对照组为2.141±0.107,两者差异无统计学意义(P＞0.05).激光共聚焦显微镜及扫描电镜可见,成骨细胞在可注射性纳米材料上可良好地增殖、生长,细胞的活性未受到材料的影响.结论:可注射性纳米人工骨具有良好的细胞相容性,可作为可注射性骨组织工程载体材料.%Objective:To explore the biocompatibility of injectable nanomaterial with rabbit osteoblasts in vitro.Methods:The marrow of New Zealand white rabbit obtained from femur and tibia was induced in the medium to obtain osteoblasts.The morphologic changes of the rabbit osteoblasts with the injectable nanomaterial in vitro were observed by laser scanning confocal microscope (LSCM) and scanning electron microscope (SEM),and the function was detected by the methods of MTT and ALP assay.Results:The osteoblasts with the injectable nanomaterial can preserve normal proliferative speed.The ALP value of the nanomaterial showed no significant difference compared with control group.It showed that the osteoblasts can proliferate and grow normally with the injectable nanomaterial,and the cellular activity was not influenced by the injectable nano artificial bone under LSCM and SEM.Conclusions:The injectable nano artificial bone has good biocompatibility and can be used as biomaterial in bone tissue engineering.