动脉粥样硬化大鼠颈总动脉糖皮质激素诱导的肿瘤坏死因子受体配体的表达及意义

摘要

目的：通过检测动脉粥样硬化大鼠颈总动脉中糖皮质激素诱导的肿瘤坏死因子受体配体（glucocorticoid‐induced tumor necrosis factor receptor ligand ，GITRL）的表达，探讨GITRL在动脉粥样硬化过程中的变化及意义。方法：建立大鼠动脉粥样硬化模型，取其颈总动脉，观察粥样硬化病变，采用免疫组织化学及蛋白质印迹（Western blotting ）法检测GITRL的表达。结果：动脉粥样硬化大鼠颈总动脉的血管壁脂质沉积明显增多；弹性纤维不完整，连续性破坏、中断。动脉粥样硬化大鼠颈总动脉血管内GIT RL的表达水平明显高于非动脉粥样硬化大鼠。结论：GIT RL作为高脂饮食后表达上调的一种血管炎性分子，有望作为预测动脉粥样硬化发生的一种独立危险因素。%Objective:To explore the change in the expression of glucocorticoid‐induced tumor necrosis factor receptor ligand (GITRL) in atherosclerotic process and its significance ,by detecting the expression of GITRL in carotid artery of rats with atherosclerosis .Methods :Rat model of atherosclerosis was established and the atherosclerotic lesions were observed . The expression of GITRL in carotid artery was analyzed by immunohistochemical method and Western blotting .Results:The lipid deposition increased significantly in carotid artery of rats with atherosclerosis . The elastic fibers were incomplete and discontinuous .The endovascular expression level of GITRL was significantly higher in the carotid artery of atherosclerosis rats than that in the carotid artery of non‐atherosclerosis rats .Conclusions :As a vascular inflammatory molecule ,produced after high‐fat diet ,GITRL may become an independent risk factor for predicting the occurrence of atherosclerosis .