实时荧光定量RT-PCR检测结直肠癌中ERCC1 mRNA的表达及其临床意义

摘要

目的 传统的影像学检查及肿瘤标志物检测对结直肠癌微转移的诊断价值有限.本研究旨在探讨ERCC1 mRNA在结直肠癌患者外周血中的表达情况及其临床意义.方法 用实时定量荧光逆转录聚合酶链反应(RTPCR)方法,对53例结直肠癌患者和21名健康人的外周血标本进行ERCC1 mRNA检测,分析其表达与肿瘤临床病理特征的关系并应用接受者操作特征曲线(ROC曲线)分析ERCC1 mRNA检测对结直肠癌的诊断价值.结果 结直肠癌组外周血ERCC1 mRNA表达水平显著高于正常对照组(P＜0.05),其表达与肿瘤淋巴结转移、血行转移和TNM分期相关(P＜0.05).ERCC1 mRNA诊断结直肠癌的ROC曲线下面积0.89,诊断界值为Ct≤32.结论 实时定量荧光RT-PCR检测ERCC1 mRNA可以更好地评估结直肠癌血道微转移,有利于判断预后.%Objective To evaluate the clinical significance of ERCCI mRNA expression in the blood of colorectal cane-er(CRC) patients. Methods The expression of ERCCI mRNA in 53 CRC patients and 21 healthy subjects was determined by real time fluorescent quantitative RT- PCR. Correlations between their expressions and clinical pathological characteristics of CRC were analyzed. Receiver operating characteristic curve( ROC curve) was used to evaluate the diagnostic value for microme-tastasis of CRC. Results The expression levels of ERCCI mRNA in CRC patients were significantly higher than those in the normal controls ( P ＜0. 05) ,and their expressions were correlated with lymph node metastasis, hematogenous metastasis and TNM staging of CRC(P＜0.05). The area under ROC curve of ERCCI mRNA in diagnosing CRC was 0. 89, the cutoff value was Ct≤32. Conclusion Detection of ERCCI mRNA by real-time fluorescent quantitative RT-PCR can assess the microme-tastasis in blood stream of colorectal cancer patients.