胃癌组织中Twist、HIF-1α表达与上皮间质转化的关系

摘要

Purpose To investigate the relationship between the expressions of Twist, HIF-1 a and clinical pathological parameters in gastric carcinoma. Explore its significance in judging the prognosis of patients. Analyze the expression of two proteins in the epithelial-mesenchymal transition phenomenon. Methods Immuohistochemistry was used to detect the expressions of Twist and HIF-lα in 120 cases of gastric carcinoma specimens and corresponding adjacent normal tissue. Results In gastric carcinoma Twist, HIF-1α., N-cad, E-cad positive rate was 59. 1% , 61. 7% , 46. 7% , 37. 5% , adjacent tissues, the positive rate was 18. 3% , 22. 5 % , 10. 8% , 91. 7%. Tumor tissues and adjacent tissues there were significant differences ( P < 0. 05 ). Patients with Twist positive expression showed higher incidence of lymph node metastasis ( P < 0. 05 ) and distant metastasis ( P < 0. 05 ) than those with Twist negative expression. HIF-1α expression was closely correlated with tumor histological type, depth of invasion, lymph node metastasis( P <0. 05 ). Patients with high expression of Twist and ( or ) HIF-1α had lower five-year survival rate than patients with negative expression. The expression of Twist and HIF-1α was significantly correlated in gastric carcinoma accompanied by low expression of E-cad and abnormal high N-cad expression ( P < 0. 05 ). Conclusions The expression of Twist and HIF-1α was closely related to the biological characteristics in gastric carcinoma, detection the expression of Twist and HIF-1α was valuable in predicting tumor prognosis, invasion and metastasis. HIF-1α promoted the phenomenon of epithelial mesenchymal transition may be by upregulating the Twist' s expression.%目的 分析Twist、HIF-1α在胃癌组织中的表达及与胃癌临床病理参数之间的相关性,探讨两者在判断患者预后中的意义及两种蛋白的表达与上皮间质转化的关系.方法 采用免疫组化SABC法检测120例胃癌标本及其对应癌旁组织中Twist、HIF-1α、E-cad、N-cad的表达,分析Twist、HIF-1α的表达与患者临床病理参数、术后生存时间之间的关系;探讨两种蛋白的表达与上皮间质转化的关系.结果 在胃癌组织中Twist、HIF-1α、N-cad、E-cad的阳性率分别为59.1%、61.7%、46.7%、37.5%,癌旁组织中的阳性率分别为18.3%、22.5%、10.8%、91.7%.4种蛋白在肿瘤组织中的表达率与癌旁组织中的表达率之比有明显差异性(P<0.05).Twist与肿瘤远处转移、淋巴结转移及浸润深度明显相关(P<0.05).HIF-1α与肿瘤的组织学分型、浸润深度、淋巴结转移呈明显相关性.Twist和(或)HIF-1α阳性的患者其五年生存率明显低于其阴性的患者.Twist、HIF-1α的表达与E-cad的低表达和N-cad的反常性高表达存在密切相关性.结论 胃癌组织中Twist、HIF-1α表达与肿瘤的生物学特征密切相关,Twist、HIF-1α的检测对预测肿瘤侵袭转移、判断预后具有一定价值.HIF-1α可能通过上调Twist表达,促进上皮间质转化现象的发生,成为促进胃腺癌浸润、转移的可能途径之一.