非小细胞肺癌中SCC-S2的表达及其在肺癌增殖侵袭的作用

摘要

目的 探讨SCC-S2在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达,以及SCC-S2对肺癌细胞增殖侵袭能力的影响.方法 采用免疫组化SP法检测146例NSCLC组织中SCC-S2蛋白表达.通过siRNA干扰SCC-S2基因表达,观察SCC-S2对细胞增殖和侵袭的影响.结果 146例NSCLC中SCC-S2阳性率为74.65%(109/146),其阳性与NSCLC的组织学类型、p-TNM分期和淋巴结转移相关.在NSCLC细胞中用siRNA干扰SCC-S2后,肺癌细胞的增殖能力与侵袭能力受到明显抑制.结论 SCC-S2在肺癌中高表达且与肺癌临床分期和淋巴结转移明显相关,SCC-S2有望成为肺癌诊断治疗的新靶点.%Purpose To study the expression of SCC-S2 in human lung cancer and its role on cell proliferation and invasion. Methods Archived tissue blocks from 146 lung cancer patients was examined using immunohistochemistry. siRNA knockdown was performed in A549 and H1299 cell lines to examine its role on proliferation and invasion. Results SCC-S2 was overexpressed in 109 of 146 ( 74. 65% ) cancer samples. SCC-S2 expression correlates with histology, p-TNM stage and nodal metastasis. siRNA depletion of SCC-S2 in A549 and H1299 cell lines inhibited cell growth and invasion. Conclusion SCC-S2 is overexpressed in lung cancer and correlates with clinical stage and nodal metastasis. SCC-S2 might serve as a therapeutic target in lung cancer.