人乳腺癌MCF-7细胞在不同HER-2水平miRNAs表达谱检测

摘要

Purpose To screen out miRNA expression profile related to HER-2 via observing the differential expression of miRNA ex-pression of human breast cancer cells MCF-7 and MCF-7/HER-2. Methods After cultivating human breast cancer cells MCF-7 and MCF-7/HER-2, the expression profile of miRNAs was detected by miRNA microarrays. Results Compared to MCF-7 cells, two hundreds and seventeen HER-2 related miRNAs were identified from breast cancer MCF-7/HER-2 cells, including 123 up-regulated and 94 down-regulated miRNAs. Of significant differentialy expressed miRNAs, hsa-miR-141 and hsa-miR-1299’s target genes have multiple biological functions. Conclusion The differentially expressed miRNAs under different lever of HER-2 in human breast cancer MCF-7 cells were obtained, which may provide a basis for studying HER-2 functions and roles in breast cancer.%目的：通过对比观察人乳腺癌细胞MCF-7和MCF-7/HER-2中微小RNA( miRNAs)的差异性表达,初步筛选出与HER-2表达相关的miRNAs表达谱。方法培养人乳腺癌细胞MCF-7和MCF-7/HER-2,利用miRNA芯片技术检测两株细胞中miR-NAs的表达。结果利用miRNA微阵列,筛选获得217种与HER-2相关的miRNAs,较正常MCF-7细胞上调的miRNAs有123个,下调的有94个。在差异有显著性的miRNAs中,hsa-miR-141和hsa-miR-1299的靶基因有多种生物学功能。结论获得人乳腺癌MCF-7细胞在不同HER-2水平miRNAs的差异表达谱,为进一步分析HER-2在乳腺癌中的作用奠定基础。