乳腺癌中LAT1和p-s6的表达及其意义

摘要

Purpose To investigate the expression and significance of type L amino acid transporter 1 (LAT1 ) and phosphorylated s6 ribosomal protein (p-s6) in breast cancer tissues and their correlation. Methods LAT1 protein and p-s6 protein were detected by immunohistochemical EnVision two step method in 178 cases of breast cancer and 78 cases of benign breast lesion, and the relationship between the expression and clinicopathological parameters was analyzed. Results The positive rate of LAT1 in breast cancer was 36.5％, which was significantly higher than that of breast benign lesion tissues (23.1 ％, P＜ 0.05 ), the positive rate of p-s6 in breast cancer tissues was33.2％, which was significantly higher than that of breast benign lesion tissues (12.8％, P＜0.05). There was a positive correlation between the expression of LAT1 protein and p-s6 protein in breast cancer tissues (r = 0.345, P＜ 0.05). The expression of LAT1 protein in breast cancer was correlated with tumor diameter, axillary lymph node metastasis, TNM staging and HER-2 level (P＜ 0.05), but not associated with the patient's age, histological grade, ER, and PR levels (P＞ 0.05). The expression of p-s6 protein was related to axillary lymph node metastasis, TNM staging, age and ER level (P＜ 0.05), but not associated with tumor diameter, histological grade, PR and HER-2 levels (P＞ 0.05 ). Multivariate analysis showed that the expres sion of LAT1 protein was related to tumor diameter and expression level of HER-2. The expression of p-s6 protein was related to axillary lymph node metastasis. Conclusion The expression of LAT1 protein and p-s6 protein in breast cancer is up-regulated, and the expression of these two proteins is positively related, which implying that LAT1 and p-s6 might play a synergistic role in the development and progression of breast cancer.%目的 探讨L型氨基酸转运子1(LAT1)和磷酸化s6(phospho-s6,p-s6)核糖体蛋白在乳腺癌组织中的表达、意义及两者的相关性.方法 采用免疫组化EnVision两步法检测178例乳腺癌组织和78例乳腺良性病变组织中LAT1蛋白和p-s6蛋白的表达,分析两者的表达与乳腺癌临床病理特征的关系.结果 乳腺癌组织中LAT1的阳性率为5％,显著高于乳腺良性病变组织(23.1％,P＜0.05);p-s6在乳腺癌组织中的阳性率为33.2％,显著高于乳腺良性病变组织(12.8％,P＜0.05);乳腺癌组织中LAT1蛋白和p-s6蛋白的表达呈正相关=0.345,P＜0.05).乳腺癌中LAT1蛋白表达与肿瘤直径、腋窝淋巴结转移、TNM分期和HER-2水平相关(P均＜0.05),与患者年龄、组织学分级、ER、PR水平均无关(P均＞0.05);p-s6蛋白表达与腋窝淋巴结转移、TNM分期、患者年龄、ER水平相关(P均＜0.05);与肿瘤直径、组织学分级、PR、HER-2水平等无关(P均＞0.05).多因素分析显蛋白表达与肿瘤直径、HER-2表达水平有关;p-s6蛋白表达与腋窝淋巴结转移有关.结论 LAT1蛋白和p-s6蛋白在乳腺癌中表达上调,且两者的表达呈正相关,提示LAT1和p-s6在乳腺癌发生、发展的过程可能发挥一定的协同作用.