泛素连接酶Hrd1与LAT1在胃癌中的表达及其意义

摘要

Objective To investigate the expressions of ubiquitin ligase Hrd1 and L⁃type amino acid transporter 1( LAT1) in gastric carcinoma tissues and their clinical significance. Methods The expression of Hrd1 and LAT1 in 62 samples of gastric carcinoma and adjacent normal tissues was detected by immunohistochemical SP method,RT⁃PCR and Western blotting. Results The positive ex⁃pression rate of Hrd1, LAT1 protein in gastric carcinoma tissues was 54�8%( 34/62) and 56�4%( 35/62) , higher than 25�0%( 5/20) and 20�0%(4/20)of adjacent normal tissue with significant differences(P<0�05). The expression of Hrd1 in gastric carcinoma tissues was only related with tumor size, and LAT1 expression was related with tumor size and clinical stage(P<0�05). RT⁃PCR showed that the quantitive expression of Hrd1 and LAT1 were 4�22±0�83 and 5�80±1�72 of grastric carcinoma tissue, higher than 1�14±0�39 and 1�32± 0�77 of adjacent normal tissue(P<0�05). Western blotting showed that the quantitive expression of Hrd1 and LAT1 were 0�855±0�153 and 0�896±0�148, higher than 0�357±0�178 and 0�439±0�136 of adjacent normal tissue(P<0�05). There was a positive correlation be⁃tween the expression of Hrd1 and LAT1 in gastric carcinoma( r=0�820,P<0�001) . Conclusion Hrd1 and LAT1 are highly expressed in gastric carcinoma. They may play an important role in the occurrence and development of gastric carcinoma.%目的：探讨泛素连接酶Hrd1与L型氨基酸转运载体1（ LAT1）在胃癌组织中的表达及临床意义。方法采用免疫组化、反转录酶⁃聚合酶链锁反应（ RT⁃PCR）及Western blotting检测62例胃癌及癌旁组织中Hrd1和LAT1的表达水平，并分析2种蛋白表达的相关性及与胃癌临床病理特征的关系。结果 Hrd1、LAT1蛋白在胃癌组织中的阳性表达率分别为54�8％（34／62）和56�4％（35／62），明显高于癌旁正常组织的25�0％（5／20）和20�0％（4／20），差异均有统计学意义（P＜0�05）。Hrd1在胃癌组织中的表达仅与肿瘤大小有关（P＜0�05），LAT1表达与肿瘤大小、临床分期有关（P＜0�05）。 RT⁃PCR检测显示，胃癌组织中Hrd1与LAT1的相对表达量分别为4�22±0�83和5�80±1�72，高于癌旁组织的1�14±0�39和1�32±0�77（P＜0�05）。 Western blotting检测显示，胃癌组织中Hrd1和LAT1的相对表达量分别为0�855±0�153和0�896±0�148，高于癌旁组织的0�357±0�178和0�439±0�136（P＜0�05）。 Hrd1与LAT1蛋白的表达水平呈正相关（r＝0�820，P＜0�001）。结论 Hrd1与LAT1在胃癌组织中的表达均高于癌旁正常组织，提示两者可能在胃癌的发生、发展中发挥重要的作用。