采用分子印迹整体柱富集-高效液相色谱法选择性分离分析了植物样品中痕量细胞分裂素的含量.结果表明,以激动素为模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,甲苯与十二醇为致孔剂,可在不锈钢柱管中原位聚合制备激动素分子印迹整体柱；与非分子印迹整体柱对比,该分子印迹整体柱能选择性富集4种细胞分裂素,具有较好的重复性和高的萃取效率；在优化的实验条件下,激动素(K)、激动素核苷(KR)、反式-玉米素(tZ)和meta-topolin(mT)的平均加标回收率分别为91.9％、80.0％、87.5％和50.2％,相对标准偏差均小于11.8％.本方法已成功地用于不同植物样品中4种细胞分裂素的分离和分析.%A method based on monolithic molecularly imprinted polymer enrichment combining with high performance liquid chromatography (mMIP-HPLC) detection was developed for the selective determination of trace cytokinins ( CTKs) in plant samples. Monolithic molecularly imprinted polymer (mMIP) column was prepared in stainless steel tube by using kinetin as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and toluene-dodecanol as the porogenic solvents. Compared with non-imprinted polymer (NIP) monolith, the prepared mMIP exhibited selective separation ability, good reproducibility and reusability, and high extraction efficiency in the separation and enrichment of the four CTKs. Under the optimized experimental conditions, mean recoveries were 91. 9% ,80. 0% ,87. 5% and 50. 2% for kinetin ( K) , kinetin glucoside ( KR) , trans-zeatin (tZ) and meto-topolin (mT) , respectively, with the corresponding RSDs less than 11. 8%. The proposed mMIP-HPLC method was successfully applied in the separation and determination of the four cytokinins in different plant samples.
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