重力场流分离系统用于聚苯乙烯颗粒的分离条件优化

摘要

重力场流分离是最简单的场流分离(gravitational flow-field fractionation,GrFFF)技术,常用于分离粒径几微米到几十微米的颗粒及生物样品.利用自组装加工的重力场流分离仪器分离3种不同粒径(3、6、20 μm)的聚苯乙烯(PS)颗粒.自制了一种混合表面活性剂,并与商品化的表面活性剂FL-70进行了比较.通过均匀设计优化流速、混合表面活性剂中聚乙二醇辛基苯基醚(Triton X-100)的质量分数、载液黏度、停流时间等分离条件,以分离度(Rs)和保留比(R)为评价指标,发现FL-70的分离效能略优于自制的混合表面活性剂,可实现3种PS颗粒的完全分离(Rs1为1.771,Rs2为2.074).结果表明该系统具有良好的分离性能.%Gravitational flow-field fractionation (GrFFF) is the simplest and cheapest fieldflow fractionation technique.GrFFF utilizes earth's gravity as external field,providing a sizebased separation.It can be applied to the separation and characterization of various micrometer-sized particles and biological samples.A GrFFF device was assembled,and three kinds of polystyrene (PS) particles with different sizes (20,6 and 3 μm) were used as the model particles.A homemade mixed surfactant was used and was compared with the surfactant FL-70.The separation conditions of GrFFF were optimized with uniform design,including flow rate,mass percentage of polyethylene glycol octylphenol ether (Triton X-100) in mixed surfactant,viscosity of carrier liquid and stop-flow time.The resolution (Rs) and retention ratio (R) were utilized as the evaluation indexes.The results suggest that the separation performance of FL-70 was slightly better than that of the mixed surfactant.Using FL-70 as the surfactant,particles of 20,6 and 3 μm were completely separated (with resolutions of 1.771 and 2.074).The results indicate that this system has good separation performance.