植物油中邻苯二甲酸酯类化合物的一步萃取方法

摘要

为实现植物油中邻苯二甲酸酯(PAEs)的现场快速检测,建立了气液微萃取(GLME)技术并与 GC-MS检测技术联用实现了植物油中 PAEs的一步萃取检测.GLME方法在气流吹扫-微注射器萃取技术的基础上建立而成.此方法定量取0.1 g植物油样品,利用 GLME在5 min内完成 PAEs的萃取、净化和浓缩,结合内标法确保了结果的准确性.在大豆油、调和油、橄榄油、香油等4种样品中添加200 μg/kg的15种PAEs,基质加标回收率为60.0%~112.3%,相对标准偏差(RSD)为0.9%~28.4%.该法操作简单、方便,准确度高,重现性好,基质干扰少,适用于现场检测和快速检测,对于保障我国食品安全、构建完整的食品安全检测体系具有重大意义.%In order to develop a fast investigation method for phthalate esters(PAEs)from vegetable oils,a gas-liquid micro-extraction(GLME)technique that combined with GC-MS was established. A vegetable oil sample(0.1 g)was directly added into the GLME device. The inte-grated process of extraction,clean-up,and concentration of PAEs was completed within 5 min. Internal standard method was applied to ensure the accuracy of the results. Soybean oil,blend oil,olive oil,and sesame oil were spiked with 200 μg/kg of a mixed 15 PAEs standard,and the ranges of the recoveries and RSDs were between 60.0% to 112.3% and 0.9% to 28.4%,respec-tively. Compared with some traditional sample pretreatment methods such as liquid-liquid extraction,liquid-liquid micro-extraction,gel permeation chromatography,this method is sim-ple and fast,with high accuracy,good repeatability and low matrix effect. This study verified the suitability of the GLME method for field detection of food products in food safety sector and exhibits great significance for the completion of food safety system in China.