采用超高效液相色谱-串联质谱技术,建立了饲料中8种镇静剂类和15种β-受体激素类药物残留的分析检测方法.样品采用乙腈-1%(体积分数)三氯乙酸水溶液(7:3,v/v)提取,目标物通过阳离子固相萃取柱净化,经Agilent Zorbax Eclipse Plus C18色谱柱(100 mm×3.0 mm,1.8 μm)分离,液相色谱-串联质谱进行检测,标准曲线内标法定量.结果表明:23种目标物在2.0~200.0 μg/L内线性关系良好(r2>0.99).在饲料样品基质中,目标化合物在5.0、10、50 μg/kg 3个加标水平下的平均回收率为75.1%~102.4%,相对标准偏差(RSD)为4.3%~14.3%(n=6).该方法净化效率高,适用范围广,可用于饲料中镇静剂类和 β-受体激素类药物残留筛查和检测.%An analysis and detection method for eight sedatives and fifteen β-receptor hor-mones in feed was established by ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS). The sample was extracted with acetonitrile-1%(v/v)trichlo-roacetic acid aqueous solution(7:3,v/v),and purified by cationic solid phase extraction col-umn. The extracts were separated on an Agilent Zorbax Eclipse Plus C18chromatographic col-umn(100 mm×3.0 mm,1.8 μm)and then quantified via internal standard method. The results showed that the linear relationships of the 23 targets were good in the range of 2.0-200.0 μg/L (r2> 0.99). The average recoveries of the targets spiked in the matrix of feed samples were within 75.1%-102.4% in three spiked levels of 5.0,10,50 μg/kg,and the relative standard deviations(RSDs)were within 4.3%-14.3%(n=6). This method with high purification effi-ciency and extensive scope of application can be applied to screening and detecting sedatives and β-receptor hormones in feed.
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