Objective To investigate recombinant human insulin-like growth factor-1 (rhIGF-1 ) on the phosphorylated serine-threonine protein kinase (pAkt) and apoptotic cells in edematous perihematomal brain regions following experimental intracerebral hemorrhage in rats. Methods A total of 78 healthy male rats were randomly assigned to a false operation group (n = 6) , a cerebral hemorrhage control group ( control group for short, n =36) and a rhIGF-1 intervention group (intervention group for short, n =36). According to 6 time points after giving intervention measures, the rats of the control and intervention groups were randomly redivided into 6 subgroups; 6h, 12h, 1 d, 2 d, 4 d, and 7 d subgroups (n-6 in each subgroup). A rat model of acute cerebral hemorrhage was induced by stereotaxic injection of collagenase type IV. RhIGF-1 (50 μg/kg) was injected via tail veins in the intervention group, once a day, until the end of the experiment. An equivalent isotonic saline was injected via tail veins each day in the sham operation and control groups. Samples were taken from each group at a specified time. An immunohistochemical method was used to observe the expression of pAkt around hematomas; deoxynucleotidyl transferase-mediated terminal deoxy-nucleotidyl transferase Dutp nick end labeling (TUNEL) technique was used to observe apoptotic cells. Results ①The neurological deficit score of the rats in the control group and intervention group reached the%目的 探讨重组人胰岛素样生长因子1(rhIGF-1)对大鼠脑出血血肿周围区磷酸化丝氨酸-苏氨酸蛋白激酶(p-Akt)及凋亡细胞的影响. 方法 健康雄性Wistar大鼠78只,随机将其分为假手术组(6只)、脑出血对照组(简称对照组,36只)、rhIGF-1干预组(简称干预组,36只).对照组和干预组大鼠按照给予干预措施后的6个时间点再随机分为6个亚组:6、12h组和1、2、4、7d组,每个亚组为6只大鼠.采用Ⅳ型胶原酶立体定位法制备大鼠急性脑出血模型.干预组经尾静脉注射rhIGF-1(50 μg/kg),1次/d,直至实验结束.假手术组和对照组每天经尾静脉注射等量等渗盐水.各组在规定的时间取材,采用免疫组化方法观察血肿周围区p-Akt的表达；脱氧核糖核苷酸末端转移酶介导的dUTP缺口末端标记技术(TUNEL)法检测凋亡细胞. 结果 ①对照组大鼠和干预组大鼠的神经功能缺损评分在术后1d或2d达到最高,此后逐渐降低,干预组大鼠的评分仅在术后第7天时低于对照组,差异均有统计学意义,P＜0.05.②假手术组大鼠仅少量表达p-Akt阳性细胞.在6h以外的其余各时间点,干预组大鼠的p-Akt阳性细胞数量均高于对照组,差异有统计学意义,P ＜0.05.③假手术组大鼠仅少量表达TUNEL阳性细胞.在除6h外的其余各时间点,干预组大鼠的TUNEL阳性细胞数量均低于对照组,差异均有统计学意义,P＜0.05. 结论 rhIGF-1能减少脑出血后的细胞凋亡,改善神经功能缺损评分,其作用可能与p-Akt的激活有关.
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