家兔血液灌流动物模型的构建和安全性评价

摘要

Objective To establish a rabbit model for hemoperfusion (HP) from peripheral vessels and to evaluate the safety of the animal model.Methods Twelve rabbits were randomly divided into two groups.For rabbits in HP group (n=6),the central ear artery at one side and ear marginal vein at the other side were cannulated with indwelling needles.Arterial indwelling needle,roller pump,hemoperfusion apparatus,triple valve and vein indwelling needle were connected by pipes.HP then begun and lasted for 2 hours with blood flow volume of 2mL/min.Blood sample were collected before HP and 2,4,8 and 24h after HP for blood routine,plasma proteins including total protein (TP),albumin (Alb) and globulin (Glb),and plasma electrolytes including Na+,K+,Ca2+and C1-assays to evaluate the safety of the rabbit HP model.Control group (n=6) were managed as same as HP group except no hemoperfusion apparatus in the circuit.Results The rabbit HP model worked well and no animal died during HP.There were no significant changes in RBC (F=0.386,P=0.548),MCV (F=0.041,P=0.844),Hb (F=0.375,P=0.554),WBC (F=0.005,P=0.943),lymphocytes (F=0.212,P=0.655),monocytes (F=0.508,P=0.492),neutrophils (F=0.026,P=0.875),platelets (F=1.270,P=0.286),mean platelet volume (F=0.037,P=0.851),Na+ (F=1.012,P=0.338),K+ (F=0.001,P=0.972),Ca2+ (F=1.812,P=0.208),TP (F=4.929,P=0.051) and Glb (F=2.641,P=0.135).However,Alb decreased after HP (F=27.490,P=0.000),and C1-increased after HP (F=11.040,P=0.008).Conclusion The rabbit HP model was established successfully,safety evaluation showed that it is suitable for further research.%目的 建立经家兔外周血管进行血液灌流(hemoperfus ion,HP)的实验动物模型并对其安全性进行评价.方法 12只健康白色新西兰家兔随机分为2组,每组6只.HP组实验动物在表面麻醉下一侧耳中央动脉置入动脉留置针,另一侧耳缘静脉置入静脉留置针,动/静脉留置针依次通过管路连接恒流泵、血液灌流器、排空气管和三通阀构成HP体外循环,以恒流泵流速2mL/min进行HP持续2h.对照组除不连接血液灌流器外,按照HP组的操作进行体外循环.HP进行期间监测家兔的生命体征,在HP开始前和HP开始后2h、4h、8h、24h,采集两组动物外周血,检测血常规、血清蛋白(总蛋白、白蛋白和球蛋白)和电解质(Na+、K+、Ca2-和C1),评价该HP动物模型的安全性. 结果 HP进行期间动物生命体征平稳,HP血流顺畅,实验期间未见动物死亡.HP组与对照组家兔红细胞计数(F0.386,P=0.548)、平均红细胞体积(F0.041,P=0.844)、血红蛋白(F=0.375,P=0.554)、白细胞计数(F=0.005,P=0.943)、淋巴细胞计数(F=0.212,P=0.655)、单核细胞计数(F=0.508,P=0.492)、中性粒细胞计数(F=0.026,P=0.875)、血小板计数(F=1.270,P=0.286)、平均血小板体积(F=0.037,P=0.851)、Na+ (F=1.012,P=0.338)、K+(F=0.001,P=0.972)、Ca2-(F=1.812,P=0.208)、血清总蛋白(F=4.929,P=0.051)和球蛋白水平(F=2.641,P=0.135)差别无统计学意义.HP组家兔血清白蛋白水平降低(F=27.490,P=0.000),C1水平升高(F=11.040,P=0.008),相对对照组差别有统计学意义. 结论 成功建立经家兔外周血管进行HP的实验动物模型,该模型安全可靠,可以满足HP实验需要.