大麦黄矮病毒GAV株系外壳蛋白基因在大肠杆菌中的高效表达

摘要

The IPTG-inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E. coli BL21 (DE3). High-level expression of the specific protein was achieved by IPTG induction. The results of SDS-PAGE and Western blottong show that the expression product which accumulates 19.5 % of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET-5a.