虫草素具有抗肿瘤、免疫调节、抗炎等多种功效.为了更好地开发蛹虫草资源,选择合适剂量的低能离子束注入蛹虫草,优化虫草素的提取工艺条件,采用紫外分光光度法检测注入前后菌株中虫草素的含量.结果表明:最佳注入剂量为2.60×10~(15)ions/cm~2,虫草素最佳的微波-超声波提取工艺为:乙醇浓度70%,提取功率200W,提取时间110 s,料液比1:240.选育出虫草素含量较高的15株菌株,最高含量达(11.924±0.063)mg/g,比原始菌株增长了近30%.%Cordycepin has the effect of anti-tumor, immunomodulation, anti-inflammation and so on.In order to make use of Cordyceps militaris better, we implanted different doses of low-energy ion beam into C.militaris, chose best cordycepin extracting technology, and determined cordycepin content in strains before and after ion beam implantation by UV spectrophotometry.Results showed that the best dose of low-energy ion beam was 2.60×10~(15) ions/cm~2, the best conditions of microwave assisted ultrasonic extraction technology were as follows: 70% ethanol as the solvent, microwave power as 200 W, extraction time as 110 s, Material - liquid ratio as 1: 240.We chose 15 strains with high-yielding cordycepin, of which cordycepin content was up to (11.924±0.063) mg/g, which was a nearly 30% increase compared with the original strain.
展开▼
