The objective of this work is to investigate how dilution rate and carbon-to-nitrogen (C/N) ratio affects lipid accumulation by Rhodosporidium toruloides AS 2.138 9 in continuous culture. Under steady-state conditions, the increase in dilution rate led to the decrease in lipid content and lipid yield. The highest lipid yield and lipid content at D=0.02 h-1 were 0.18 g lipid/g sugar and 57.1%, respectively, while the highest lipid productivity and biomass productivity were obtained at D=0.14 h-1. The increase in C/N ratio led to the increase in lipid content. The highest lipid content of 38% was obtained at C/N=237. The highest lipid yield of 0.12 g lipid/g sugar was obtained at C/N=92. However, the highest lipid productivity of 0.12 g/(L-h) was obtained at C/N=32. No significant changes were observed in terms of fatty acid composition of the lipid produced under different C/N ratios, and these three fatty acids, palmitic acid, stearic acid and oleic acid, took over 85% in all samples.%采用恒化培养的方法,考察了稀释率(D)和碳氮比(mol/mol)对圆红冬孢酵母Rhodosporidium toruloides AS 2.138 9积累油脂的影响.结果表明:稀释率增大,油脂含量和油脂得率降低.在D=0.02 h-1时油脂得率最大,为0.18g油/g糖；D=0.14 h-1时油脂生成速率最大,为0.09 g/(L·h).碳氮比增大,油脂含量略有增加.在C/N=92时油脂得率最大,为0.12 g油/g糖；C/N=32时油脂生成速率最大,为0.13 g/(L·h).碳氮比对油脂的脂肪酸组成影响不明显,油脂的棕榈酸、硬脂酸和油酸总含量超过85％.
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