拟南芥Leafy Cotyledon 2的表达提高了小球藻Chlorella sorokiniana的油脂含量

摘要

油脂含量是影响微藻产业化生产生物柴油的因素之一.基因工程的方法是培育高产藻株的一个重要手段.Leafy Cotyledon 2 (LEC2)在拟南芥中是调节种子成熟及油脂积累的重要转录因子,在藻类植物中尚无相关的报道.本研究从拟南芥中获取LEC2基因,构建表达载体pCIMBIA 1300-35 s-GFP-ATLEC2,通过基因枪介导法转入小球藻C.sorokiniana.经过PCR、RT-PCR、Western blotting分析,筛选出一株转ATLEC2基因藻株.对总脂肪酸含量的分析发现,转ATLEC2基因藻株的脂肪酸含量比原始藻株提高了1倍且没有明显影响生长.以上结果表明,ATLEC2能促进小球藻油脂的积累.%The low lipid content is one of the major bottlenecks to realize the industrialization of the algae biodiesel.Improvement of lipid content through global regulation to get high-yield generating algae is a good strategy.Leafy Cotyledon 2 (LEC2) is an important transcription factor for seed maturation and oil accumulation in Arabidopsis.However,there are few reports regarding adoption of LEC2 for lipid accumulation until now.In this study,LEC2 from Arabidopsis was cloned into the plant expression vector pCIMBIA1300 and transformed into C.sorokiniana through particle bombardment.One recombinant was screened by PCR,RT-PCR and Western blot analyses.Compared with the wild type one,the total lipid content in the recombinant increased one fold,which did not show effect on cell growth,indicating that LEC2 can efficiently enhance the lipid accumulation in C.sorokiniana.