小麦淀粉废水发酵生产丁醇

摘要

In order to reduce the production cost of butanol fermentation,low concentration of wheat starch wastewater was used to produce butanol, acetone and ethanol with Clostridium acetobutylicum. Medium was optimized according to the composition of starch wastewater. Effects of cassava concentration、nitrogen source and concentration, inorganic salt and the method of raw material pretreatment were investigated. Addition of 70 g/L cassava, 2 g/L yeast extract powder and 1 g/L K2 HPO4 to low concentration wastewater gave better performance,the yields of butanol and total solvent in flask were able to arrive at 14�72 and 22�65 g/L,respectively. Meanwhile,the yields of butanol and total solvent in a 7⁃L fermentor could achieved 13�51 and 23�13 g/L. Through extractive fermentation, the yields of butanol and total solvent reached 15�13 and 29�38 g/L,respectively.%为降低丁醇发酵的生产成本，以工业废弃物小麦淀粉废水为辅料，在分析废水组成的基础之上补加适当营养成分发酵生产丁醇。选取对丁醇发酵影响最大的木薯浓度、N源种类、N源浓度、无机盐和原料处理方式等因素分别进行考察。结果表明：对于低浓度废水，添加70 g／L木薯、2 g／L酵母粉、1 g／L K2 HPO4，原料糊化水解，能够获得较好的摇瓶发酵效果，丁醇和总溶剂产量最高可分别达到14�72和22�65 g／L。在7 L发酵罐水平上，丁醇和总溶剂产量也分别能达到13�51和23�13 g／L。最终，利用生物柴油进行萃取发酵，其溶剂水平得到进一步提高，丁醇和总溶剂产量可分别达到15�13和29�38 g／L。