餐厨垃圾和市政污泥配料比对厌氧产甲烷特性及微生物多样性的影响

摘要

在(38±0.5)℃的温度下,研究不同配料比对餐厨垃圾和市政污泥混合厌氧消化过程中系统参数及微生物群落多样性的影响,设定餐厨垃圾和市政污泥的挥发性固体的质量比为1:0、0:1、1:1、1:2和2:1,并采用高通量测序技术分析厌氧消化过程中微生物组成的多样性.结果表明:质量比为1:1的体系混合厌氧消化时,产甲烷效果最佳,单位质量的挥发性固体含量的产甲烷量可达到232.86 L/kg,挥发性固体含量的去除率达46.09％.各个混合比例体系中的优势物种为拟杆菌门(Bacteroidetes)和甲烷八叠球菌属(Methanosarcina).在餐厨垃圾中添加市政污泥混合厌氧消化能够提高微生物群落的多样性,促进菌种间的协同作用,进而提升产气效率.%In order to investigate the effects of food waste ( FW )/municipal sludge ( MS ) ratio on the reactor performance and microbial community diversity at the mesophilic temperature (38±0. 5)℃,lab-scale experiments were carried out at five volatile solid ( VS) ratios of FW:MS at 1:0,0:1,1:1,1:2, and 2:1. High-throughput sequencing technique was also used to reveal the microbial community composition of the anaerobic digestion system. Results showed that food waste ( FW)/municipal sludge ( MS ) ratio had complex influences on methane yield and microbial community. The accumulative maximum methane yield ( VS ) 232. 86 L/kg and removal of volatile solid content ( 46. 09％) were obtained when food waste ( FW)/municipal sludge ( MS) ratio was controlled at 1:1. High-throughput sequencing indicated that Bacteroidetes and Methanosarcina dominated in whole process of all the mixed systems. In conclusion,co-digestion system could significantly improve methane production by increasing microbial community diversity and synergistic effects between different species.