人尿红细胞生成素和血栓调节素的纯化研究

摘要

目的从人尿中分离纯化红细胞生成素(EPO)和血栓调节素(TM)。方法用超滤浓缩、离子交换层析及亲和层析等方法从新鲜人尿中分别纯化EPO和TM。结果从2 000 kg新鲜人尿中分别纯化得到了4.47 mg EPO和9.92 mg TM，得率分别为31.9%和25.0%，分子量分别为(38.6±1.0)kD和(60±1.4)kD；等电点分别为3.60±0.02和3.70±0.02；TM富含Glu、Ala和Gly，毛细管区带电泳分析高度均一，有关生化参数与文献报道基本一致。结论该工艺可获得纯度较高的EPO和TM。%Purpose　The aim is to isolate and purify the erythropoietin(EPO)and thrombomodulin(TM) from human urine.Methods　Purifying and isolating human urine with means of filtration and concentration,ion-exchange chromatography, affinity chromatography and so on.Results　4.47 mg EPO and 9.92 mg TM were obtained from 2 000 kg human urine.Their molecular weights were(38.6±1.0)kD and (60±1.4)kD erespectively, the isoelectric point, 3.60±0.02 and 3.70±0.02, yield, 31.9% and 25.0%.The purified EPO and TM was shown to be highly homogeneous by capillary zone-electrophoresis(CZE),abundant Glu，Ala and Gly include on TM. Conclusion　The high purity EPO and TM was obtained.