毛细管电泳测定微量氨基酸柱上样品堆积法研究

摘要

Purpose　The aim is to develop a method for the analysis of micro-scale amino acids in biological samples.Methods　After having been extracted and dried,the residues were dissolved in a running buffer or a distilled water and acetonitrile.The samples were loaded into column for 5,25 or 50s at a hydrodynamic injection and were separated by capillary electrophoresis.The effect of different dissolvent on the sample stacking was observed.Results　A 100-fold improvement in the amount of material that can be injected into a capillary column without loss of resolution was shown.Conclusion　The marked effect of sample stacking was achieved by the using distilled water and acetonitrile(1∶1,v/v) as dissolvent.%目的建立一种柱上样品堆积法测定微量氨基酸的方法。方法分别用运行缓冲液或用水和乙腈重组经萃取吹干的样品，压力法进样5、25和50 s，高效毛细管电泳法定量分析，观察用不同溶剂重组对柱上样品堆积效果的影响。结果该法提高微量氨基酸检测灵敏度100倍并保持柱效不变。结论用水和乙腈(1∶1)作溶剂重组残留样品的方法获得最佳的柱上堆积效果。