氧化苦参碱对内毒素作用的人牙周膜细胞增殖和分泌肿瘤坏死因子-α的影响

摘要

目的 观察氧化苦参碱(OMT)对内毒素(LPS)作用下的人牙周膜细胞(HPDLC)增殖和分泌肿瘤坏死因子-α( TNF-α)的影响.方法 体外原代培养HPDLC,采用MTT法观察OMT对LPS作用的HPDLC增殖活性的影响,采用酶联免疫方法检测培养上清液中的TNF-α水平.结果100 μg/mL LPS可抑制HPDLC的增殖活性并刺激其分泌TNF-α,而在加入LPS同时分别给予1～25μg/mL OMT能在一定程度上恢复HPDLC的增殖活性并抑制LPS刺激的HPDLC TNF-α的分泌.结论 OMT对LPS作用的HPDLC具有保护作用.%Purpose To investigate the effects of oxymatrine ( OMT) on the proliferation and expression of tumor necrosis factor-α ( TNF-α) in human periodontal ligament cells ( HPDLC) treated with lipopolysaccharide(LPS). Methods HPDLC were primarily cultured in vitro. MTT colorimetric assay was performed to detect the cell proliferation. ELISA was applied to detect the level of TNF-α in the culture rnsupernatant. Results The proliferation was remarkably inhibited by incubation of HPDLC with 100 μg/ mL LPS of E. coli, while the level of TNF-α was significantly increased. After adding different concentrations of OMT from 1 to 25 μg/mL, the proliferation was greatly improved and the production of TNF-α was decreased. Conclusion OMT may have protective effects on HPDLC treated with LPS.