雷帕霉素对胶质瘤患者瘤体解旋酶RECQ1表达的影响研究

摘要

目的：探讨雷帕霉素对脑胶质瘤患者瘤体解旋酶RECQ1（Tumor helicase RECQ1）表达的影响。方法选取河北医科大学第二医院神经外科收治的脑胶质瘤患者50例，随机分为2组，其中对照组25例，予常规手术治疗；实验组25例，先予以雷帕霉素胶囊，1 mg，1次/天口服，连续服用14天，停药后1周行手术治疗。术中取胶质瘤组织样本，采用逆转录聚合酶链反应（RT-PCR）技术和Western blot技术检测瘤体解旋酶RECQ1 mRNA和蛋白质表达。结果对照组患者胶质瘤组织瘤体解旋酶RECQ1 mRNA表达较实验组胶质瘤组织明显升高，对照组光密度值为（1.657±0.748），而实验组光密度值为（1.059±0.894），统计分析结果显示，差异有统计学意义（P＜0.05）；所有组织中瘤体解旋酶RECQ1蛋白均有表达，但实验组患者的胶质瘤组织中的瘤体解旋酶RECQ1蛋白表达（0.952±0.021）明显低于对照组（1.211±0.024），差异有统计学意义（P＜0.05）。结论雷帕霉素胶囊能够降低解旋酶RECQ1 mRNA表达，能够抑制脑胶质肿瘤细胞DNA的复制，有效杀灭癌细胞，控制脑胶质瘤的发展，改善预后，对临床具有指导意义，值得临床推广。%Objective To investigate the effects of rapamycin on the expression of glioma patients tumor helicase RECQ1.Methods 50 glioma patients admitted to the department of neurosurgery in second hospital of hebei medical university were selected and randomly divided into 2 groups,25 patients in control group,were treated with routine admission surgical treatment;25 cases in the experimental group,firstly were given rapamycin capsule 1 mg,1 times/day orally,took 14 days in a row,and had surgical treatment after stopping drug a week.Glioma tissue samples were taken during the operation,mRNA and protein expression of tumor helicase RECQ1 were detected by reverse transcriptase polymerase chain reaction(RT-PCR)and Western blot.Results Glioma tumor helicase RECQ1 mRNA expression in the control group increased more significantly than experimental group,the optical density value in control group was(1.657 ±0.748),while the experimental group optical density value was(1.059 ±0.894),and the difference was statistically significant(P<0.05 );all organizations had the expression of tumor helicase RECQ1 protein,but gliomas tumor helicase RECQ1 protein expression in the experimental group patients(0.952 ±0.021)was significantly lower than that in the control group(1.211 ±0.024),and the difference was statistically significant(P<0.05).Conclusion Rapamycin capsule could reduce the expression of mRNA helicase RECQ1,inhibit DNA glial tumor cells of brain replication,effectively kill cancer cells,control the the progress of brain glioma,and improve prognosis,worth clinical promotion.